Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR
Descripción del Articulo
Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performe...
| Autores: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/2514 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/2514 https://doi.org/10.2217/fmb-2019-0280 |
| Nivel de acceso: | acceso abierto |
| Materia: | stomach neoplasms eosin Helicobacter pylori hematoxylin histopathology immunohistochemistry PCR http://purl.org/pe-repo/ocde/ford#3.04.02 |
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CONCYTEC-Institucional |
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4689 |
| dc.title.none.fl_str_mv |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| title |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| spellingShingle |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR A Castaneda C. stomach neoplasms eosin Helicobacter pylori hematoxylin histopathology immunohistochemistry PCR http://purl.org/pe-repo/ocde/ford#3.04.02 |
| title_short |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| title_full |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| title_fullStr |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| title_full_unstemmed |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| title_sort |
Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR |
| author |
A Castaneda C. |
| author_facet |
A Castaneda C. Castillo M. Sanchez J. Casavilca S. A Bernabe L. Suarez N. Chavez I. Ruiz E. Tello K. R Villa M. Zevallos R. Montenegro P. Dias-Neto E. Landa-Baella M. Taxa L. |
| author_role |
author |
| author2 |
Castillo M. Sanchez J. Casavilca S. A Bernabe L. Suarez N. Chavez I. Ruiz E. Tello K. R Villa M. Zevallos R. Montenegro P. Dias-Neto E. Landa-Baella M. Taxa L. |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
A Castaneda C. Castillo M. Sanchez J. Casavilca S. Sanchez J. A Bernabe L. Suarez N. Chavez I. Ruiz E. Tello K. R Villa M. Zevallos R. Montenegro P. Dias-Neto E. Landa-Baella M. Taxa L. |
| dc.subject.none.fl_str_mv |
stomach neoplasms |
| topic |
stomach neoplasms eosin Helicobacter pylori hematoxylin histopathology immunohistochemistry PCR http://purl.org/pe-repo/ocde/ford#3.04.02 |
| dc.subject.es_PE.fl_str_mv |
eosin Helicobacter pylori hematoxylin histopathology immunohistochemistry PCR |
| dc.subject.ocde.none.fl_str_mv |
http://purl.org/pe-repo/ocde/ford#3.04.02 |
| description |
Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results. © 2020 Future Medicine Ltd.. All rights reserved. |
| publishDate |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/2514 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.2217/fmb-2019-0280 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85091266055 |
| url |
https://hdl.handle.net/20.500.12390/2514 https://doi.org/10.2217/fmb-2019-0280 |
| identifier_str_mv |
2-s2.0-85091266055 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Future Microbiology |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Future Medicine Ltd. |
| publisher.none.fl_str_mv |
Future Medicine Ltd. |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
| instname_str |
Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
| reponame_str |
CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883125561196544 |
| spelling |
Publicationrp06454600rp06447600rp06449600rp06445600rp06449600rp06451600rp06456600rp06448600rp05755600rp06450600rp06452600rp06457600rp06444600rp06446600rp06455600rp06453600A Castaneda C.Castillo M.Sanchez J.Casavilca S.Sanchez J.A Bernabe L.Suarez N.Chavez I.Ruiz E.Tello K.R Villa M.Zevallos R.Montenegro P.Dias-Neto E.Landa-Baella M.Taxa L.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2020https://hdl.handle.net/20.500.12390/2514https://doi.org/10.2217/fmb-2019-02802-s2.0-85091266055Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results. © 2020 Future Medicine Ltd.. All rights reserved.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengFuture Medicine Ltd.Future Microbiologyinfo:eu-repo/semantics/openAccessstomach neoplasmseosin-1Helicobacter pylori-1hematoxylin-1histopathology-1immunohistochemistry-1PCR-1http://purl.org/pe-repo/ocde/ford#3.04.02-1Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCRinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/2514oai:repositorio.concytec.gob.pe:20.500.12390/25142024-05-30 16:08:55.462http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="73a2ce4f-1dff-4609-b9b2-75cfd721ef44"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR</Title> <PublishedIn> <Publication> <Title>Future Microbiology</Title> </Publication> </PublishedIn> <PublicationDate>2020</PublicationDate> <DOI>https://doi.org/10.2217/fmb-2019-0280</DOI> <SCP-Number>2-s2.0-85091266055</SCP-Number> <Authors> <Author> <DisplayName>A Castaneda C.</DisplayName> <Person id="rp06454" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Castillo M.</DisplayName> <Person id="rp06447" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Sanchez J.</DisplayName> <Person id="rp06449" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Casavilca S.</DisplayName> <Person id="rp06445" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Sanchez J.</DisplayName> <Person id="rp06449" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>A Bernabe L.</DisplayName> <Person id="rp06451" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Suarez N.</DisplayName> <Person id="rp06456" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Chavez I.</DisplayName> <Person id="rp06448" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ruiz E.</DisplayName> <Person id="rp05755" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Tello K.</DisplayName> <Person id="rp06450" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>R Villa M.</DisplayName> <Person id="rp06452" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Zevallos R.</DisplayName> <Person id="rp06457" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Montenegro P.</DisplayName> <Person id="rp06444" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Dias-Neto E.</DisplayName> <Person id="rp06446" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Landa-Baella M.</DisplayName> <Person id="rp06455" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Taxa L.</DisplayName> <Person id="rp06453" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Future Medicine Ltd.</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>stomach neoplasms</Keyword> <Keyword>eosin</Keyword> <Keyword>Helicobacter pylori</Keyword> <Keyword>hematoxylin</Keyword> <Keyword>histopathology</Keyword> <Keyword>immunohistochemistry</Keyword> <Keyword>PCR</Keyword> <Abstract>Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results. © 2020 Future Medicine Ltd.. All rights reserved.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
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13.394457 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
