Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population

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We thank the staff at the Instituto de Medicina Tropical Alexander von Humboldt for their valuable assistance in their attention to the HTLV-1-infected people. This work was supported by the Directorate-General for Development Cooperation of the Belgian Government through the Framework Agreement wit...

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Detalles Bibliográficos
Autores: Best, I, Lopez, G, Talledo, M, MacNamara, A, Verdonck, K, Gonzalez, E, Tipismana, M, Asquith, B, Gotuzzo, E, Vanham, G, Clark, D
Formato: artículo
Fecha de Publicación:2011
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/1148
Enlace del recurso:https://hdl.handle.net/20.500.12390/1148
https://doi.org/10.1089/aid.2011.0029
Nivel de acceso:acceso abierto
Materia:Adult
Aged
Amino Acid Sequence
Carrier State
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oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/1148
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
title Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
spellingShingle Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
Best, I
Adult
Aged
Amino Acid Sequence
Carrier State
title_short Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
title_full Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
title_fullStr Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
title_full_unstemmed Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
title_sort Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
author Best, I
author_facet Best, I
Lopez, G
Talledo, M
MacNamara, A
Verdonck, K
Gonzalez, E
Tipismana, M
Asquith, B
Gotuzzo, E
Vanham, G
Clark, D
author_role author
author2 Lopez, G
Talledo, M
MacNamara, A
Verdonck, K
Gonzalez, E
Tipismana, M
Asquith, B
Gotuzzo, E
Vanham, G
Clark, D
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Best, I
Lopez, G
Talledo, M
MacNamara, A
Verdonck, K
Gonzalez, E
Tipismana, M
Asquith, B
Gotuzzo, E
Vanham, G
Clark, D
dc.subject.en.fl_str_mv Adult
Aged
Amino Acid Sequence
Carrier State
topic Adult
Aged
Amino Acid Sequence
Carrier State
description We thank the staff at the Instituto de Medicina Tropical Alexander von Humboldt for their valuable assistance in their attention to the HTLV-1-infected people. This work was supported by the Directorate-General for Development Cooperation of the Belgian Government through the Framework Agreement with the Institute of Tropical Medicine of Antwerp and through the Flemish Interuniversity Council (VLIR), and by the Peruvian National Board for Science and Technology (CONCYTEC).
publishDate 2011
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/1148
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1089/aid.2011.0029
dc.identifier.isi.none.fl_str_mv 296659900009
url https://hdl.handle.net/20.500.12390/1148
https://doi.org/10.1089/aid.2011.0029
identifier_str_mv 296659900009
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv AIDS Research and Human Retroviruses
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.en.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844883012480663552
spelling Publicationrp03244600rp03248600rp03243600rp03247600rp01235500rp03242600rp03249600rp03245600rp01238500rp03246600rp01998500Best, ILopez, GTalledo, MMacNamara, AVerdonck, KGonzalez, ETipismana, MAsquith, BGotuzzo, EVanham, GClark, D2024-05-30T23:13:38Z2024-05-30T23:13:38Z2011https://hdl.handle.net/20.500.12390/1148https://doi.org/10.1089/aid.2011.0029296659900009We thank the staff at the Instituto de Medicina Tropical Alexander von Humboldt for their valuable assistance in their attention to the HTLV-1-infected people. This work was supported by the Directorate-General for Development Cooperation of the Belgian Government through the Framework Agreement with the Institute of Tropical Medicine of Antwerp and through the Flemish Interuniversity Council (VLIR), and by the Peruvian National Board for Science and Technology (CONCYTEC).HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic and progressive disorder caused by the human T-lymphotropic virus type 1 (HTLV-1). In HTLV-1 infection, a strong cytotoxic T cell (CTL) response is mounted against the immunodominant protein Tax. Previous studies carried out by our group reported that increased IFN-γ enzyme-linked immunospot (ELISPOT) responses against the region spanning amino acids 161 to 233 of the Tax protein were associated with HAM/TSP and increased HTLV-1 proviral load (PVL). An exploratory study was conducted on 16 subjects with HAM/TSP, 13 asymptomatic carriers (AC), and 10 HTLV-1-seronegative controls (SC) to map the HAM/TSP-associated CTL epitopes within Tax region 161–233. The PVL of the infected subjects was determined and the specific CTL response was evaluated with a 6-h incubation IFN-γ ELISPOT assay using peripheral blood mononuclear cells (PBMCs) stimulated with 16 individual overlapping peptides covering the Tax region 161–233. Other proinflammatory and Th1/Th2 cytokines were also quantified in the supernatants by a flow cytometry multiplex assay. In addition, a set of human leukocyte antigen (HLA) class I alleles that bind with high affinity to the CTL epitopes of interest was determined using computational tools. Univariate analyses identified an association between ELISPOT responses to two new CTL epitopes, Tax 173–185 and Tax 181–193, and the presence of HAM/TSP as well as an increased PVL. The HLA-A*6801 allele, which is predicted to bind to the Tax 181–193 peptide, was overpresented in the HAM/TSP patients tested.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengMary Ann LiebertAIDS Research and Human Retrovirusesinfo:eu-repo/semantics/openAccessAdultAgedAmino Acid SequenceCarrier StateShort Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Populationinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#20.500.12390/1148oai:repositorio.concytec.gob.pe:20.500.12390/11482025-09-25 08:32:45.42http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="a8e3952f-3c89-41f7-b460-d71cf42af1c2"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population</Title> <PublishedIn> <Publication> <Title>AIDS Research and Human Retroviruses</Title> </Publication> </PublishedIn> <PublicationDate>2011</PublicationDate> <DOI>https://doi.org/10.1089/aid.2011.0029</DOI> <ISI-Number>296659900009</ISI-Number> <Authors> <Author> <DisplayName>Best, I</DisplayName> <Person id="rp03244" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Lopez, G</DisplayName> <Person id="rp03248" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Talledo, M</DisplayName> <Person id="rp03243" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>MacNamara, A</DisplayName> <Person id="rp03247" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Verdonck, K</DisplayName> <Person id="rp01235" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Gonzalez, E</DisplayName> <Person id="rp03242" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Tipismana, M</DisplayName> <Person id="rp03249" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Asquith, B</DisplayName> <Person id="rp03245" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Gotuzzo, E</DisplayName> <Person id="rp01238" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Vanham, G</DisplayName> <Person id="rp03246" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Clark, D</DisplayName> <Person id="rp01998" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Mary Ann Liebert</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Adult</Keyword> <Keyword>Aged</Keyword> <Keyword>Amino Acid Sequence</Keyword> <Keyword>Carrier State</Keyword> <Abstract>HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic and progressive disorder caused by the human T-lymphotropic virus type 1 (HTLV-1). In HTLV-1 infection, a strong cytotoxic T cell (CTL) response is mounted against the immunodominant protein Tax. Previous studies carried out by our group reported that increased IFN-γ enzyme-linked immunospot (ELISPOT) responses against the region spanning amino acids 161 to 233 of the Tax protein were associated with HAM/TSP and increased HTLV-1 proviral load (PVL). An exploratory study was conducted on 16 subjects with HAM/TSP, 13 asymptomatic carriers (AC), and 10 HTLV-1-seronegative controls (SC) to map the HAM/TSP-associated CTL epitopes within Tax region 161–233. The PVL of the infected subjects was determined and the specific CTL response was evaluated with a 6-h incubation IFN-γ ELISPOT assay using peripheral blood mononuclear cells (PBMCs) stimulated with 16 individual overlapping peptides covering the Tax region 161–233. Other proinflammatory and Th1/Th2 cytokines were also quantified in the supernatants by a flow cytometry multiplex assay. In addition, a set of human leukocyte antigen (HLA) class I alleles that bind with high affinity to the CTL epitopes of interest was determined using computational tools. Univariate analyses identified an association between ELISPOT responses to two new CTL epitopes, Tax 173–185 and Tax 181–193, and the presence of HAM/TSP as well as an increased PVL. The HLA-A*6801 allele, which is predicted to bind to the Tax 181–193 peptide, was overpresented in the HAM/TSP patients tested.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.924177
Short Communication An Interferon-gamma ELISPOT Assay with Two Cytotoxic T Cell Epitopes Derived from HTLV-1 Tax Region 161-233 Discriminates HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Patients from Asymptomatic HTLV-1 Carriers in a Peruvian Population
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

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