Injured coliforms in drinking water of buildings of Lima Cercado

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To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique...

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Detalles Bibliográficos
Autores: Vergaray, Germán, Méndez, Carmen, Béjar, Vilma, Morante, Hilda, Heredia, Vidalina
Formato: artículo
Fecha de Publicación:2007
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - IIGEO
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/535
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/535
Nivel de acceso:acceso abierto
Materia:Injured coliforms
fecal contamination
Coliformes injuriados
contaminación fecal
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oai_identifier_str oai:ojs.csi.unmsm:article/535
network_acronym_str 1682-3087
network_name_str Revista UNMSM - IIGEO
dc.title.none.fl_str_mv Injured coliforms in drinking water of buildings of Lima Cercado
Coliformes injuriados en el agua de bebida de edificios
title Injured coliforms in drinking water of buildings of Lima Cercado
spellingShingle Injured coliforms in drinking water of buildings of Lima Cercado
Vergaray, Germán
Injured coliforms
fecal contamination
Coliformes injuriados
contaminación fecal
title_short Injured coliforms in drinking water of buildings of Lima Cercado
title_full Injured coliforms in drinking water of buildings of Lima Cercado
title_fullStr Injured coliforms in drinking water of buildings of Lima Cercado
title_full_unstemmed Injured coliforms in drinking water of buildings of Lima Cercado
title_sort Injured coliforms in drinking water of buildings of Lima Cercado
dc.creator.none.fl_str_mv Vergaray, Germán
Méndez, Carmen
Béjar, Vilma
Morante, Hilda
Heredia, Vidalina
author Vergaray, Germán
author_facet Vergaray, Germán
Méndez, Carmen
Béjar, Vilma
Morante, Hilda
Heredia, Vidalina
author_role author
author2 Méndez, Carmen
Béjar, Vilma
Morante, Hilda
Heredia, Vidalina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Injured coliforms
fecal contamination
Coliformes injuriados
contaminación fecal
topic Injured coliforms
fecal contamination
Coliformes injuriados
contaminación fecal
dc.description.none.fl_txt_mv To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demonstrated that 15% of the water samples was insuitable for the human consumption. Of the considered suitable 85, 29,41% (25) was containing IC. In the suitable samples using the conventional methods, 60% (9) had between 0.1 and 0.5 ppm of RFC and in the considered insuitable samples for having IC, 88% (22) had between 0.1 and 1.0 ppm. Relation was not demonstrated between pH and unsuitable samples. Among the IC were identifi ed: Escherichia coli, Enterobacter aerogenes, E. cloacae, Enterobacter sp, Citrobacter freundii, Citrobacter spp and Klebsiella sp. The drinking water considered suitable by the Peruvian normativity can be contaminated by intestinal microorganisms and constitute a risk for the health; the residual free chlorine between 0.1 and 1.0 ppm does not guarantee the elimination of the intestinal bacteria.
Para detectar Coliformes injuriados (CI) en el agua considerada apta para el consumo humano según la normatividad peruana, se tomaron y procesaron 100 muestras de agua de edifi cios del distrito de Lima-Cercado. Se determinó el cloro libre residual (CLR) mediante la técnica DPD y el pH usando varillas indicadoras Merck. Para el recuento de bacterias heterotrófi cas (BH) se empleó la técnica de spread plate; para cuantifi car Coliformes totales (CT) y Coliformes fecales (CF) se empleó la técnica de tubos múltiples y de fi ltración por membrana. Para recuperar y enumerar CT y CF injuriados se utilizó el medio m-T7. Para la especifi cación bacteriana las pruebas estándar y el sistema API 20E. Para la detección de CI se utilizaron las muestras que resultaron negativas para CT y CF y/o tenían un recuento de BH igual o inferior a 500 UFC/mL. Empleando los métodos convencionales se demostró que el 15% de las muestras de agua eran inaptas para el consumo humano. De las 85 consideradas aptas, el 29.41% (25) contenía CI. En las muestras inaptas empleando los métodos convencionales, el 60% (9) tenía entre 0,1 y 0,5 ppm de CLR y en las muestras consideradas inaptas por tener CI, el 88% (22) tenía entre 0,1 y 1,0 ppm. No se demostró relación entre el pH y las muestras inaptas. Entre los CI se identifi caron: Escherichia coli, Enterobacter aerogenes, E. cloacae, Enterobacter sp, Citrobacter freundii, Citrobacter spp y Klebsiella sp. El agua para consumo humano considerada apta por la normatividad peruana puede estar contaminada con microorganismos intestinales y constituir un riesgo para la salud; el CLR entre 0,1 y 1,0 ppm no garantiza la eliminación de las bacterias intestinales.
description To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demonstrated that 15% of the water samples was insuitable for the human consumption. Of the considered suitable 85, 29,41% (25) was containing IC. In the suitable samples using the conventional methods, 60% (9) had between 0.1 and 0.5 ppm of RFC and in the considered insuitable samples for having IC, 88% (22) had between 0.1 and 1.0 ppm. Relation was not demonstrated between pH and unsuitable samples. Among the IC were identifi ed: Escherichia coli, Enterobacter aerogenes, E. cloacae, Enterobacter sp, Citrobacter freundii, Citrobacter spp and Klebsiella sp. The drinking water considered suitable by the Peruvian normativity can be contaminated by intestinal microorganisms and constitute a risk for the health; the residual free chlorine between 0.1 and 1.0 ppm does not guarantee the elimination of the intestinal bacteria.
publishDate 2007
dc.date.none.fl_str_mv 2007-07-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/535
10.15381/iigeo.v10i19.535
url https://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/535
identifier_str_mv 10.15381/iigeo.v10i19.535
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/535/455
dc.rights.none.fl_str_mv Derechos de autor 2007 Germán Vergaray, Carmen R. Méndez, Vilma R. Béjar, Hilda Y. Morante, Vidalina Heredia
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2007 Germán Vergaray, Carmen R. Méndez, Vilma R. Béjar, Hilda Y. Morante, Vidalina Heredia
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Facultad de Ingeniería Geológica, Minera, Metalurgica y Geográfica
publisher.none.fl_str_mv Facultad de Ingeniería Geológica, Minera, Metalurgica y Geográfica
dc.source.none.fl_str_mv Revista del Instituto de Investigación de la Facultad de Ingeniería Geológica, Minera, Metalúrgica y Geográfica; Vol 10 No 19 (2007); 51-54
Revista del Instituto de Investigación de la Facultad de Ingeniería Geológica, Minera, Metalúrgica y Geográfica; Vol. 10 Núm. 19 (2007); 51-54
1682-3087
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spelling Injured coliforms in drinking water of buildings of Lima CercadoColiformes injuriados en el agua de bebida de edificiosVergaray, GermánMéndez, CarmenBéjar, VilmaMorante, HildaHeredia, VidalinaInjured coliformsfecal contaminationColiformes injuriadoscontaminación fecalTo detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demonstrated that 15% of the water samples was insuitable for the human consumption. Of the considered suitable 85, 29,41% (25) was containing IC. In the suitable samples using the conventional methods, 60% (9) had between 0.1 and 0.5 ppm of RFC and in the considered insuitable samples for having IC, 88% (22) had between 0.1 and 1.0 ppm. Relation was not demonstrated between pH and unsuitable samples. Among the IC were identifi ed: Escherichia coli, Enterobacter aerogenes, E. cloacae, Enterobacter sp, Citrobacter freundii, Citrobacter spp and Klebsiella sp. The drinking water considered suitable by the Peruvian normativity can be contaminated by intestinal microorganisms and constitute a risk for the health; the residual free chlorine between 0.1 and 1.0 ppm does not guarantee the elimination of the intestinal bacteria.Para detectar Coliformes injuriados (CI) en el agua considerada apta para el consumo humano según la normatividad peruana, se tomaron y procesaron 100 muestras de agua de edifi cios del distrito de Lima-Cercado. Se determinó el cloro libre residual (CLR) mediante la técnica DPD y el pH usando varillas indicadoras Merck. Para el recuento de bacterias heterotrófi cas (BH) se empleó la técnica de spread plate; para cuantifi car Coliformes totales (CT) y Coliformes fecales (CF) se empleó la técnica de tubos múltiples y de fi ltración por membrana. Para recuperar y enumerar CT y CF injuriados se utilizó el medio m-T7. Para la especifi cación bacteriana las pruebas estándar y el sistema API 20E. Para la detección de CI se utilizaron las muestras que resultaron negativas para CT y CF y/o tenían un recuento de BH igual o inferior a 500 UFC/mL. Empleando los métodos convencionales se demostró que el 15% de las muestras de agua eran inaptas para el consumo humano. De las 85 consideradas aptas, el 29.41% (25) contenía CI. En las muestras inaptas empleando los métodos convencionales, el 60% (9) tenía entre 0,1 y 0,5 ppm de CLR y en las muestras consideradas inaptas por tener CI, el 88% (22) tenía entre 0,1 y 1,0 ppm. No se demostró relación entre el pH y las muestras inaptas. Entre los CI se identifi caron: Escherichia coli, Enterobacter aerogenes, E. cloacae, Enterobacter sp, Citrobacter freundii, Citrobacter spp y Klebsiella sp. El agua para consumo humano considerada apta por la normatividad peruana puede estar contaminada con microorganismos intestinales y constituir un riesgo para la salud; el CLR entre 0,1 y 1,0 ppm no garantiza la eliminación de las bacterias intestinales.Facultad de Ingeniería Geológica, Minera, Metalurgica y Geográfica2007-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/53510.15381/iigeo.v10i19.535Revista del Instituto de Investigación de la Facultad de Ingeniería Geológica, Minera, Metalúrgica y Geográfica; Vol 10 No 19 (2007); 51-54Revista del Instituto de Investigación de la Facultad de Ingeniería Geológica, Minera, Metalúrgica y Geográfica; Vol. 10 Núm. 19 (2007); 51-541682-30871561-0888reponame:Revista UNMSM - IIGEOinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/535/455Derechos de autor 2007 Germán Vergaray, Carmen R. Méndez, Vilma R. Béjar, Hilda Y. Morante, Vidalina Herediahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2020-07-07T18:25:20Zmail@mail.com -
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