Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
Descripción del Articulo
Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability....
Autores: | , , , , , |
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Formato: | artículo |
Fecha de Publicación: | 2020 |
Institución: | Universidad Nacional Mayor de San Marcos |
Repositorio: | Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
Lenguaje: | español |
OAI Identifier: | oai:ojs.csi.unmsm:article/17186 |
Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186 |
Nivel de acceso: | acceso abierto |
Materia: | Ornithobacterium rhinotracheale antibiotic resistance genotyping ERIC-PCR resistencia a antibióticos genotipificación |
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Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017Caracterización fenotípica y genotípica de Ornithobacterium rhinotracheale procedentes de aves de corral con cuadros clínicos respiratorios en el Perú entre 2015 y 2017Cotaquispe Nalvarte, Rony YuniorRodríguez Bailón, JorgeKoga Yanagui, IsabelTinoco Romero, RobertDe La Flor Montaubán, IreneManchego Sayán, AlbertoOrnithobacterium rhinotrachealeantibiotic resistancegenotypingERIC-PCROrnithobacterium rhinotrachealeresistencia a antibióticosgenotipificaciónERIC-PCROrnithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin.Ornithobacterium rhinotracheale, al igual que otras bacterias asociadas a cuadros de orden respiratorio, viene siendo sometida a una exigente presión de selección debido al uso de desinfectantes y antibióticos, lo cual ha venido generando cepas multidrogo resistentes, así como un incremento en la variabilidad genética. El objetivo del estudio fue caracterizar 36 aislados de O. rhinotracheale provenientes de aves de corral de cinco regiones del Perú, mediante un perfil de ocho antibióticos (fosfomicina, amoxicilina, ciprofloxacina, enrofloxacina, norfloxacina, sulfatrimetopim, doxiciclina, oxitetraciclina), y genotipificación por técnicas de ERIC-PCR. Los resultados indican la presencia de multidrogo resistencia de 2 a 6 antibióticos en 94.4% (n=34) de las cepas y 6.6% (n=2) cepas fueron resistentes a un antibiótico, siendo la resistencia más común a la fosfomicina (77.8%) seguido del sulfatrimetopim (75.0%). Se encontraron 11 perfiles genéticos diferenciados (A-K), siendo el perfil D más frecuente en los aislados (55.6%). Los resultados sugieren la presencia de cepas de O. rhinotracheale con moderada variabilidad y perfiles de resistencia a los antibiótico fosfomicina y sulfatrimetopim.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-02-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1718610.15381/rivep.v30i4.17186Revista de Investigaciones Veterinarias del Perú; Vol 30 No 4 (2019); 1734-1742Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 4 (2019); 1734-17421682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186/14633Derechos de autor 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayánhttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T18:10:12Zmail@mail.com - |
dc.title.none.fl_str_mv |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 Caracterización fenotípica y genotípica de Ornithobacterium rhinotracheale procedentes de aves de corral con cuadros clínicos respiratorios en el Perú entre 2015 y 2017 |
title |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 |
spellingShingle |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 Cotaquispe Nalvarte, Rony Yunior Ornithobacterium rhinotracheale antibiotic resistance genotyping ERIC-PCR Ornithobacterium rhinotracheale resistencia a antibióticos genotipificación ERIC-PCR |
title_short |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 |
title_full |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 |
title_fullStr |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 |
title_full_unstemmed |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 |
title_sort |
Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017 |
dc.creator.none.fl_str_mv |
Cotaquispe Nalvarte, Rony Yunior Rodríguez Bailón, Jorge Koga Yanagui, Isabel Tinoco Romero, Robert De La Flor Montaubán, Irene Manchego Sayán, Alberto |
author |
Cotaquispe Nalvarte, Rony Yunior |
author_facet |
Cotaquispe Nalvarte, Rony Yunior Rodríguez Bailón, Jorge Koga Yanagui, Isabel Tinoco Romero, Robert De La Flor Montaubán, Irene Manchego Sayán, Alberto |
author_role |
author |
author2 |
Rodríguez Bailón, Jorge Koga Yanagui, Isabel Tinoco Romero, Robert De La Flor Montaubán, Irene Manchego Sayán, Alberto |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ornithobacterium rhinotracheale antibiotic resistance genotyping ERIC-PCR Ornithobacterium rhinotracheale resistencia a antibióticos genotipificación ERIC-PCR |
topic |
Ornithobacterium rhinotracheale antibiotic resistance genotyping ERIC-PCR Ornithobacterium rhinotracheale resistencia a antibióticos genotipificación ERIC-PCR |
dc.description.none.fl_txt_mv |
Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin. Ornithobacterium rhinotracheale, al igual que otras bacterias asociadas a cuadros de orden respiratorio, viene siendo sometida a una exigente presión de selección debido al uso de desinfectantes y antibióticos, lo cual ha venido generando cepas multidrogo resistentes, así como un incremento en la variabilidad genética. El objetivo del estudio fue caracterizar 36 aislados de O. rhinotracheale provenientes de aves de corral de cinco regiones del Perú, mediante un perfil de ocho antibióticos (fosfomicina, amoxicilina, ciprofloxacina, enrofloxacina, norfloxacina, sulfatrimetopim, doxiciclina, oxitetraciclina), y genotipificación por técnicas de ERIC-PCR. Los resultados indican la presencia de multidrogo resistencia de 2 a 6 antibióticos en 94.4% (n=34) de las cepas y 6.6% (n=2) cepas fueron resistentes a un antibiótico, siendo la resistencia más común a la fosfomicina (77.8%) seguido del sulfatrimetopim (75.0%). Se encontraron 11 perfiles genéticos diferenciados (A-K), siendo el perfil D más frecuente en los aislados (55.6%). Los resultados sugieren la presencia de cepas de O. rhinotracheale con moderada variabilidad y perfiles de resistencia a los antibiótico fosfomicina y sulfatrimetopim. |
description |
Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186 10.15381/rivep.v30i4.17186 |
url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186 |
identifier_str_mv |
10.15381/rivep.v30i4.17186 |
dc.language.none.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186/14633 |
dc.rights.none.fl_str_mv |
Derechos de autor 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayán http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Derechos de autor 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayán http://creativecommons.org/licenses/by-nc-sa/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol 30 No 4 (2019); 1734-1742 Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 4 (2019); 1734-1742 1682-3419 1609-9117 reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
reponame_str |
Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
collection |
Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
instname_str |
Universidad Nacional Mayor de San Marcos |
instacron_str |
UNMSM |
institution |
UNMSM |
repository.name.fl_str_mv |
-
|
repository.mail.fl_str_mv |
mail@mail.com |
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1701389176465784832 |
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13.95948 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).