Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017

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Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability....

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Autores: Cotaquispe Nalvarte, Rony Yunior, Rodríguez Bailón, Jorge, Koga Yanagui, Isabel, Tinoco Romero, Robert, De La Flor Montaubán, Irene, Manchego Sayán, Alberto
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/17186
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186
Nivel de acceso:acceso abierto
Materia:Ornithobacterium rhinotracheale
antibiotic resistance
genotyping
ERIC-PCR
resistencia a antibióticos
genotipificación
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spelling Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017Caracterización fenotípica y genotípica de Ornithobacterium rhinotracheale procedentes de aves de corral con cuadros clínicos respiratorios en el Perú entre 2015 y 2017Cotaquispe Nalvarte, Rony YuniorRodríguez Bailón, JorgeKoga Yanagui, IsabelTinoco Romero, RobertDe La Flor Montaubán, IreneManchego Sayán, AlbertoOrnithobacterium rhinotrachealeantibiotic resistancegenotypingERIC-PCROrnithobacterium rhinotrachealeresistencia a antibióticosgenotipificaciónERIC-PCROrnithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin.Ornithobacterium rhinotracheale, al igual que otras bacterias asociadas a cuadros de orden respiratorio, viene siendo sometida a una exigente presión de selección debido al uso de desinfectantes y antibióticos, lo cual ha venido generando cepas multidrogo resistentes, así como un incremento en la variabilidad genética. El objetivo del estudio fue caracterizar 36 aislados de O. rhinotracheale provenientes de aves de corral de cinco regiones del Perú, mediante un perfil de ocho antibióticos (fosfomicina, amoxicilina, ciprofloxacina, enrofloxacina, norfloxacina, sulfatrimetopim, doxiciclina, oxitetraciclina), y genotipificación por técnicas de ERIC-PCR. Los resultados indican la presencia de multidrogo resistencia de 2 a 6 antibióticos en 94.4% (n=34) de las cepas y 6.6% (n=2) cepas fueron resistentes a un antibiótico, siendo la resistencia más común a la fosfomicina (77.8%) seguido del sulfatrimetopim (75.0%). Se encontraron 11 perfiles genéticos diferenciados (A-K), siendo el perfil D más frecuente en los aislados (55.6%). Los resultados sugieren la presencia de cepas de O. rhinotracheale con moderada variabilidad y perfiles de resistencia a los antibiótico fosfomicina y sulfatrimetopim.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-02-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1718610.15381/rivep.v30i4.17186Revista de Investigaciones Veterinarias del Perú; Vol 30 No 4 (2019); 1734-1742Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 4 (2019); 1734-17421682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186/14633Derechos de autor 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayánhttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T18:10:12Zmail@mail.com -
dc.title.none.fl_str_mv Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
Caracterización fenotípica y genotípica de Ornithobacterium rhinotracheale procedentes de aves de corral con cuadros clínicos respiratorios en el Perú entre 2015 y 2017
title Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
spellingShingle Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
Cotaquispe Nalvarte, Rony Yunior
Ornithobacterium rhinotracheale
antibiotic resistance
genotyping
ERIC-PCR
Ornithobacterium rhinotracheale
resistencia a antibióticos
genotipificación
ERIC-PCR
title_short Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
title_full Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
title_fullStr Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
title_full_unstemmed Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
title_sort Phenotypic and genotypic characterization of Ornithobacterium rhinotracheale from poultry with respiratory clinical signs in Peru between 2015 and 2017
dc.creator.none.fl_str_mv Cotaquispe Nalvarte, Rony Yunior
Rodríguez Bailón, Jorge
Koga Yanagui, Isabel
Tinoco Romero, Robert
De La Flor Montaubán, Irene
Manchego Sayán, Alberto
author Cotaquispe Nalvarte, Rony Yunior
author_facet Cotaquispe Nalvarte, Rony Yunior
Rodríguez Bailón, Jorge
Koga Yanagui, Isabel
Tinoco Romero, Robert
De La Flor Montaubán, Irene
Manchego Sayán, Alberto
author_role author
author2 Rodríguez Bailón, Jorge
Koga Yanagui, Isabel
Tinoco Romero, Robert
De La Flor Montaubán, Irene
Manchego Sayán, Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ornithobacterium rhinotracheale
antibiotic resistance
genotyping
ERIC-PCR
Ornithobacterium rhinotracheale
resistencia a antibióticos
genotipificación
ERIC-PCR
topic Ornithobacterium rhinotracheale
antibiotic resistance
genotyping
ERIC-PCR
Ornithobacterium rhinotracheale
resistencia a antibióticos
genotipificación
ERIC-PCR
dc.description.none.fl_txt_mv Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin.
Ornithobacterium rhinotracheale, al igual que otras bacterias asociadas a cuadros de orden respiratorio, viene siendo sometida a una exigente presión de selección debido al uso de desinfectantes y antibióticos, lo cual ha venido generando cepas multidrogo resistentes, así como un incremento en la variabilidad genética. El objetivo del estudio fue caracterizar 36 aislados de O. rhinotracheale provenientes de aves de corral de cinco regiones del Perú, mediante un perfil de ocho antibióticos (fosfomicina, amoxicilina, ciprofloxacina, enrofloxacina, norfloxacina, sulfatrimetopim, doxiciclina, oxitetraciclina), y genotipificación por técnicas de ERIC-PCR. Los resultados indican la presencia de multidrogo resistencia de 2 a 6 antibióticos en 94.4% (n=34) de las cepas y 6.6% (n=2) cepas fueron resistentes a un antibiótico, siendo la resistencia más común a la fosfomicina (77.8%) seguido del sulfatrimetopim (75.0%). Se encontraron 11 perfiles genéticos diferenciados (A-K), siendo el perfil D más frecuente en los aislados (55.6%). Los resultados sugieren la presencia de cepas de O. rhinotracheale con moderada variabilidad y perfiles de resistencia a los antibiótico fosfomicina y sulfatrimetopim.
description Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic profiles (A-K) were found, being the most frequent the D profile in the isolates (55.6%). The results suggest the presence of O. rhinotracheale strains with moderate variability and antibiotic resistance profiles fosfomycin and sulfatrimetropin.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186
10.15381/rivep.v30i4.17186
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186
identifier_str_mv 10.15381/rivep.v30i4.17186
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17186/14633
dc.rights.none.fl_str_mv Derechos de autor 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayán
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Rony Yunior Cotaquispe Nalvarte, Jorge Rodríguez Bailón, Isabel Koga Yanagui, Robert Tinoco Romero, Irene De La Flor Montaubán, Alberto Manchego Sayán
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol 30 No 4 (2019); 1734-1742
Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 4 (2019); 1734-1742
1682-3419
1609-9117
reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
reponame_str Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
collection Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
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repository.mail.fl_str_mv mail@mail.com
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