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artículo
Bacterial resistance to antibiotics has become an important public health issue that involves human medicine, veterinary medicine, food and environmental safety in Perú. This work, developed in a private laboratory located in the city of  Lima - Peru,  focused on the phenotypic and genotypic detection of Escherichia coli (Escherich, 1885) strains that produce extended spectrum β-lactamase enzymes (βLEE), isolated in poultry. A total of 185 isolated bird samples of E. coli from 26 intensive breeding companies located in Peru were evaluated. The results reflected that 38.4% of strains with the presence of ESBL confirmed phenotypically; Likewise, genes were expressed from total DNA by polymerase chain reaction (PCR) obtaining 33.5% between the blaTEM, blaSHV and blaCTX-M-1 genes and 4.9% of E. coli strains producing βLEE phenotypic, no gene bla determined. As a conclusion, the...
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artículo
La resistencia bacteriana frente a los antibióticos ha tomado importancia en los últimos años como un gran problema en la salud pública, que involucra tanto a la medicina humana, como a la medicina veterinaria y a la seguridad alimentaria como ambiental en Perú. Los objetivos de este trabajo fueron detectar fenotípica y genotípica cepas de Escherichia coli (Escherich, 1885) productoras de enzimas β-Lactamasas de espectro extendido (βLEE), aisladas en aves de abasto en un laboratorio privado ubicado en la ciudad de Lima, Perú. Se evaluaron 185 aislamientos de E. coli procedentes de aves de 26 empresas de crianza intensiva ubicadas en Perú. Los resultados reflejan en 38,4% de las cepas con presencia de enzimas βLEE confirmadas fenotípicamente; asimismo se expresó genes a partir de ADN total por reacción en cadena de la polimerasa (PCR) obteniendo 33,5% entre los genes blaTEM...
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artículo
Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic p...
4
artículo
Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic p...