Se ha demostrado que los antibióticos aminoglucósidos interactúan con varias moléculas de ARN al unirse a regiones con estructuras secundarias. Varios reportes indican que la neomicina B puede inhibir la replicación del VIH y otros virus ARN. El genoma rotaviral consiste de 11 segmentos de ARN de doble hebra, la replicación viral emplea ARN cadena positiva (ARNm) como templado para la síntesis de ARN de cadena negativa. Se ha determinado que señales existentes en cis del ARNm son necesarias para la replicación al involucrar secuencias y conformaciones esenciales para el reconocimiento de la polimerasa viral. Debido a que se requieren dos pasos para la síntesis de ARN para la morfogénesis del virus, se ha estudiado los efectos de neomicina B y otros aminoglucósidos en la transcripción y/o replicación del genoma viral. Los resultados demuestran que neomicina B a concentracion...

The aim of this study was the genotyping of Ornithobacterium rhinotracheale. For this, 36 strains were identified by molecular testing and genotyping was performed by Multilocus Sequence Typing (MLST). The tests identified the type sequences: N.T.1, N.T2 and N.T3, different from the NCBI database and 19 different alleles with 229 polymorphic sites. In addition, 32 strains belonged to the common ST1 genotype. The findings identified at least four genotypes in chickens from various regions of Peru.

Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic p...

Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic p...