The aim of this study was the phenotyping of 60 isolates of Gallibacterium sp through a profile of six families of antibiotics and their genotyping by ERIC-PCR, REP-PCR and BOXAIR techniques. It was found that 98.3% of the isolates are resistant to at least one family of antimicrobials, and 11.7% were resistant to all six families. The REP-PCR presented 45 genotypes encoded from R1 to R45, the ERIC-PCR presented 44 genotypes encoded from E1 to E44 and BOXAIR presented 56 genotypes encoded from B1 to B56, dispersed in the departments of Lima, Ica and Libertad (Peru). The study allowed to typify Gallibacterium sp and the combined use of genotypic and phenotypic methods allowed a more precise discrimination between potentially different isolates with moderate genetic diversity in the cities of Lima, Libertad and Ica. 

El objetivo del presente trabajo fue la fenotipificación 60 aislados de Gallibacterium sp mediante un perfil de seis familias de antibióticos y su genotipificación por técnicas de ERIC-PCR, REP-PCR y BOXAIR. Se encontró que el 98.3% de los aislados resisten al menos a una familia de antimicrobianos, y el 11.7% fueron resistentes a las seis familias. La REP-PCR presentó 45 genotipos codificados de R1 hasta R45, la ERIC-PCR presentó 44 genotipos codificados de E1 hasta E44 y la BOXAIR presentó 56 genotipos codificados de B1 hasta B56, dispersos en los departamentos de Lima, Ica y Libertad (Perú). El estudio permitió tipificar Gallibacterium sp y el uso combinado de métodos genotípicos y fenotípicos permitió una discriminación más precisa entre aislamientos potencialmente diferentes con moderada diversidad genética en las ciudades de Lima, Libertad y Ica.

The aim of this study was the genotyping of Ornithobacterium rhinotracheale. For this, 36 strains were identified by molecular testing and genotyping was performed by Multilocus Sequence Typing (MLST). The tests identified the type sequences: N.T.1, N.T2 and N.T3, different from the NCBI database and 19 different alleles with 229 polymorphic sites. In addition, 32 strains belonged to the common ST1 genotype. The findings identified at least four genotypes in chickens from various regions of Peru.