Diez microsatélites para alpacas y llamas fueron usados para evaluar parentesco en 47 alpacas registradas de la Estación Experimental IVITA - Maranganí, provenientes de la provincia de Canchis (Cusco - Perú). El análisis se llevo a cabo utilizando dos metodologías: secuenciador automático (ABI 377 DNA sequencersâ) y técnica de tinción con nitrato de plata. Los microsatélites fueron amplificados en tres reacciones de PCR múltiple y diez reacciones de PCR simple. Los 10 microsatélites fueron polimórficos para ambas metodologías. El número de alelos vario entre 4 y 20, las frecuencias alélicas y probabilidad de exclusión (PE) fueron calculadas utilizando el software Cervus 2.0. Todos los loci, a excepción de dos, se encontraron dentro de los rangos publicados por Lang et al. (1996) y Penedo et al. (1998). La probabilidad de exclusión acumulada para los 10 loci de 0.9999....

The aim of this study was the genotyping of Ornithobacterium rhinotracheale. For this, 36 strains were identified by molecular testing and genotyping was performed by Multilocus Sequence Typing (MLST). The tests identified the type sequences: N.T.1, N.T2 and N.T3, different from the NCBI database and 19 different alleles with 229 polymorphic sites. In addition, 32 strains belonged to the common ST1 genotype. The findings identified at least four genotypes in chickens from various regions of Peru.

Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic p...

Ornithobacterium rhinotracheale, like other bacteria associated with respiratory conditions, has been subjected to a demanding selection pressure due to the use of disinfectants and antibiotics, which has been generating resistant multidrug strains, as well as an increase in its genetic variability. The objective of the study was to characterize 36 O. rhinotracheale isolates from poultry from five regions of Peru by a profile of eight antibiotics (fosfomycin, amoxicillin, ciprofloxacin, enrofloxacin, norfloxacin, sulfatrimetropin, doxycycline, oxytetracycline), and genotyping by ERIC-PCR techniques. The results indicate the presence of multidrug resistance of 2 to 6 antibiotics in 94.4% (n = 34) of the strains and 6.6% (n = 2) strains were resistant to 1 antibiotic, being the most common resistance to fosfomycin (77.8 %) and sulfatrimetropin (75.0%). At least, 11 differentiated genetic p...