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1
tesis de grado
This final report aimed to determine the relationship between personal finances and financial well-being in the members of the Association of Motorcycle Drivers of the Province of Ucayali Contamana 2023, worked with a method applied with a non-experimental design, selecting a population of 250 members from which a sample of 152 was taken from whom a questionnaire of questions was used for data collection. The results show that the level of personal finances is regular (35%), since they sometimes keep track of their expenses on a weekly basis. However, they tend to prioritize the payment of their outstanding debts and in most cases identify a surplus of their income to allocate it to savings. Regarding the financial well-being of the members, it is fair (33%), because they are not prepared for unforeseen expenses and almost never have money left over at the end of the month. Finally, it w...
2
artículo
Phospholipases A2 (PLA2 ) from snake venom are enzymes with highly variety of biological effects, due to their different isoforms and some may be myotoxins. This research aimed was to purify, characterize and evaluate the myotoxic activity of an isoform of acid PLA2 (BaPer-PLA2a). For purification, in DEAE Sephadex-A50, Sephadex-G75 and an automated medium pressure system-NGC were used. BaPer-PLA2a had a specific activity of 34.1 U/ mg and a MW of ~ 14.5 kDa by SDS-PAGE, non-reducing conditions. From venom were obtained total RNA, to synthesis of cDNA and an amplified of ~ 480 bp. A mature protein of 124 amino acids was deduced from the cDNA sequence with a pI (4.41), being an acidic isoform, likewise presented primary structure with conserved regions and residues His48, Asp49 and Tyr52 identified in the catalytic center. Additionally, the structural theoretical model has an identity gre...