La síntesis de nanopartículas de plata se realiza mediante métodos químicos, físicos y biológicos, dentro de este último se encuentra la síntesis verde, la cual ha ido adquiriendo relevante importancia, donde se utilizan extractos vegetales, los cuales aportan biorreductores a través de sus principios activos o metabolitos secundarios para obtener nanopartículas de plata biogénicas. El presente trabajo de investigación se llevó a cabo en el laboratorio LAPCINANO perteneciente a la Universidad Nacional de San Agustín de Arequipa y tiene como objetivo sintetizar nanopartículas de plata (AgNPs) a partir del extracto hidroalcohólico y la fracción flavonoide (fracción etanol) de la especie vegetal Lepechinia meyenii y evaluar su actividad antibacteriana y anticancerígena. Para ello, se utilizó como precursor AgNO3 1 mM y como reductores el extracto hidroalcohólico de Lepe...

“In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25...

“In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25...