The biological properties of mesenchymal stem cells (MSCs) from synovial fluid from horse limb joints were analysed. The samples were characterized by their peculiarity of adhering to the plastic of the culture flasks. The electromagnetic field technique (MACS) and the surface biomarker STRO-1 were used to optimize their isolation by enriching mesenchymal precursor cells (MSC-LS). To determine the expression of multipotency genes, total RNA was extracted and M-MLV reverse transcriptase was used for the cDNA, which served as a template for qPCR (quantitative PCR). qPCR was performed using the BioRad system with Sybr Green quantification. The multipotency genes quantified were OCT4 and NANOG. The cells selected using MACS with the surface biomarker STRO-1 (CMM-LS STRO-1) proliferate more rapidly than the CMM-LS obtained without purification. In addition, they showed a greater predispositio...

Equine seminal plasma was used in a lactose-EDTA extender for cryopreservation of equine epididymal sperm. Twelve pairs of testicles of slaughtered horses were used. Epididymides were separated and washed applying the retrograde technique to obtain sperm by injecting 10 ml of lactose-EDTA extender through the vas deferens. Samples with more than 30% motility were used. Samples were diluted 1: 1 with the lactose-EDTAextender seminal plasma and filled into 0.5 ml straws at a concentration of 386.3 x 106, frozen in liquid nitrogen and stored for 10 days. The values for fresh and thawed samples were: motility: 43.3 and 16.4% (p<0.05), viability: 48.3 and 40.5%, normal morphology: 67.1 and 56.5%, and integrity of the plasma membrane (HOS): 48.3 and 45.5%.

Equine seminal plasma was used in a lactose-EDTA extender for cryopreservation of equine epididymal sperm. Twelve pairs of testicles of slaughtered horses were used. Epididymides were separated and washed applying the retrograde technique to obtain sperm by injecting 10 ml of lactose-EDTA extender through the vas deferens. Samples with more than 30% motility were used. Samples were diluted 1: 1 with the lactose-EDTAextender seminal plasma and filled into 0.5 ml straws at a concentration of 386.3 x 106, frozen in liquid nitrogen and stored for 10 days. The values for fresh and thawed samples were: motility: 43.3 and 16.4% (p<0.05), viability: 48.3 and 40.5%, normal morphology: 67.1 and 56.5%, and integrity of the plasma membrane (HOS): 48.3 and 45.5%.

El desarrollo del proyecto en la primera etapa involucró el uso de laboratorios, de microbiología y el laboratorio piloto de investigación, debido a la necesidad de compartir equipos para diferentes procesos que requería el cultivo de Lactobacillus plantarum. En la segunda etapa y en esta última etapa solo se trabajó en el laboratorio piloto. Se han desarrollado los trabajos de aislamiento y cultivo microbiológico, y de preparación de los sistemas de generación de campo magnético alterno de hasta 53 kHz. Se ha logrado obtener la influencia del campo magnético variable en el crecimiento del Lactobacillus, observando que las muestras sometidas a campo en tiempos cortos y mediana frecuencia han producido un aumento de colonias aproximadamente en un 70% más, comparado con las muestras control.

El desarrollo del proyecto en la primera etapa involucró el uso de laboratorios, de microbiología y el laboratorio piloto de investigación, debido a la necesidad de compartir equipos para diferentes procesos que requería el cultivo de Lactobacillus plantarum. En la segunda etapa y en esta última etapa solo se trabajó en el laboratorio piloto. Se han desarrollado los trabajos de aislamiento y cultivo microbiológico, y de preparación de los sistemas de generación de campo magnético alterno de hasta 53 kHz. Se ha logrado obtener la influencia del campo magnético variable en el crecimiento del Lactobacillus, observando que las muestras sometidas a campo en tiempos cortos y mediana frecuencia han producido un aumento de colonias aproximadamente en un 70% más, comparado con las muestras control.