El objetivo del presente estudio fue evaluar el efecto del acetato de plomo Pb(CH COO) sobre la 3 2 hiperactivación espermática in vitro en espermatozoides humanos. En este estudio prospectivo, se midieron los parámetros del esperma, tales como la concentración de los espermatozoides, motilidad, viabilidad y morfología de acuerdo con los criterios de la OMS. Además de estos parámetros estándar, se observó el efecto del acetato de plomo utilizando tres concentraciones -1 -1 -1(0.6µg.dL ; 6µg.dL y 66µg.dL ) sobre la hiperactivación in vitro de espermatozoides humanos. Los resultados muestran una disminución de la vitalidad, dando una CL para espermatozoides 50 -1 no hiperactivados igual a 5.58µg.dL y para el caso de espermatozoides hiperactivados fue de -1196.16 µg.dL , dando los valores de NOEC y LOEC para el porcentaje de mortalidad y -1 -1porcentaje de motilidad para amb...

Equine seminal plasma was used in a lactose-EDTA extender for cryopreservation of equine epididymal sperm. Twelve pairs of testicles of slaughtered horses were used. Epididymides were separated and washed applying the retrograde technique to obtain sperm by injecting 10 ml of lactose-EDTA extender through the vas deferens. Samples with more than 30% motility were used. Samples were diluted 1: 1 with the lactose-EDTAextender seminal plasma and filled into 0.5 ml straws at a concentration of 386.3 x 106, frozen in liquid nitrogen and stored for 10 days. The values for fresh and thawed samples were: motility: 43.3 and 16.4% (p<0.05), viability: 48.3 and 40.5%, normal morphology: 67.1 and 56.5%, and integrity of the plasma membrane (HOS): 48.3 and 45.5%.

Equine seminal plasma was used in a lactose-EDTA extender for cryopreservation of equine epididymal sperm. Twelve pairs of testicles of slaughtered horses were used. Epididymides were separated and washed applying the retrograde technique to obtain sperm by injecting 10 ml of lactose-EDTA extender through the vas deferens. Samples with more than 30% motility were used. Samples were diluted 1: 1 with the lactose-EDTAextender seminal plasma and filled into 0.5 ml straws at a concentration of 386.3 x 106, frozen in liquid nitrogen and stored for 10 days. The values for fresh and thawed samples were: motility: 43.3 and 16.4% (p<0.05), viability: 48.3 and 40.5%, normal morphology: 67.1 and 56.5%, and integrity of the plasma membrane (HOS): 48.3 and 45.5%.