The authors acknowledge the CONCYTEC from Peru for the attribution of a master grant to one of us to realize part of the studies.

The authors gratefully acknowledge the financial assistance of CONCYTEC from Peru and DSF-IRD (JEAI) from France. We express our thanks to members of the Chayahuitas community who were willing to share with us their knowledge about medicinal plants. The authors are grateful to Candy Ruiz from LID-UPCH, for technical assistance in plants extractions.

The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of Hermetia illucens larvae. Extract from C. multifidum culture broth supernatant showed moderate activity against a strain of methicillin-resistant Staphylococcus aureus (MRSA). Bioguided isolation of the extract resulted in the characterization of six ?-pyrone derivatives (1-6) and one diketopiperazine (7). Of these compounds, 5,6-dihydro-4-methoxy-6-(1-oxopentyl)-2Hpyran- 2-one (4) showed the greatest activity (IC50 = 11.4 ± 0.7 ?g/mL and MIC = 62.5 ?g/ mL) against MRSA. © 2019 Correa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribut...