To develop a new product pharmaceutical industries, use analytical methods to quantify the active principle fast and reliable. The validation was determined if the method is reliable and reproducible, for this reason the present work validated an analytical method by high resolution liquid chromatography to quantify sennosides in chocolate bars, the type of research was descriptive with simple random sampling. The chromatographic conditions at 274 nm, mobile phase with a flow gradient ranging from 0.4 to 1, 5 mL / minute and solvent, composed to phosphate buffer pH 3.5 and Acetonitrile, injection volume 40 μL. The evaluated validation parameters confirmed specificity, linearity to concentrations of 0.07; 0.11; 0.15; 0.19 and 0.23 mg / mL with a correlation coefficient of 0.9996, precision with a coefficient of variation less than 2 %, accuracy of 98.6 % and robustness after 24 hours wit...

El selenio es un oligoelemento esencial para la salud humana y la deficiencia de este nutriente provoca con frecuencia trastornos como diabetes, enfermedad de Keshan, disfunción tiroidea y limitación cognitiva. El selenio orgánico y iónico son altamente biodisponibles, pero el selenio elemental lo es difícilmente. En el desarrollo de un producto farmacéutico es necesario utilizar un método analítico específico para cuantificar el principio activo en una formulación. La cromatografía líquida de alta resolución posee diversas aplicaciones y al ser automatizada permite realizar análisis de forma rápida y eficiente y ser aplicado en uso rutinario en el análisis de control de calidad y estabilidad del medicamento. Las condiciones cromatográficas empleadas son columna L1 Octadecil silano 300 mm x 3,9 mm x 10 µm, temperatura del horno a 30 ºC, longitud de onda a 330 nm, fase ...

The objective of the study was to determine the antioxidant activity in the lyophilized and atomized powder of Ipomoea batata L. by the DPPH and FRAP methods, in the variety from the Piura area known as “purple sweet potato”. Two drying methods were used, spray and lyophilization. To determine the antioxidant capacity, the DPPH and FRAP methods were used. The solubility test was carried out with water, ethanol and methanol. The results were the values of 23.23 μmol Trolox equivalents / g for DPPH and 20.18 μmol Trolox equivalents / g for FRAP in the lyophilized sample and values of 5.24 μmol Trolox equivalents / g for DPPH and 4.96 μmol Trolox equivalents / g for FRAP in the atomized sample. In conclusion, the results show that the antioxidant capacity of the variety of Ipomoea Batatas L. (Purple Sweet Potato) presents a great difference in the results according to the drying met...