Diarrhea continues to be a health problem worldwide, especially in developing countries where it is responsible for 1,87 million deaths / year in children under 5 years of age. In Peru it has been shown that diarrheogenic Escherichia coli are an important cause of diarrhea in children. The objective of this research was to determine the frequency of enteropathogenic Escherichia coli (EPEC) in children under 5 years of age in a pediatric hospital in Lima, Peru. A total of 70 diarrheic stool samples were collected from children with clinical symptoms of acute diarrhea at the Pediatric Emergency Hospital, during the months of June to December 2015. Isolation and biochemical identification was performed by co-cultures and conventional biochemical tests, the identification of enteropathogenic Escherichia coli (EPEC) was performed by serotyping using polyvalent and monovalent antisera to the O...

La diarrea continúa siendo un problema de salud en todo el mundo especialmente en los países en vías de desarrollo donde es responsable de 1,87 millones de muertes/año en niños menores de cinco años. En el Perú se ha demostrado que Escherichia coli diarreogénicas son causa importante de diarrea en niños. Esta investigación tuvo como objetivo determinar la frecuencia de Escherichia coli enteropatógena (EPEC) en niños menores de cinco años en un hospital pediátrico de Lima Perú. Se colectaron 70 muestras de heces diarreicas de niños con síntomas clínicos de diarrea aguda del Hospital de Emergencias Pediátricas, durante los meses junio a diciembre del 2015. El aislamiento e identificación bioquímica se realizó por coprocultivos y pruebas bioquímicas convencionales, la identificación de Escherichia coli enteropatogena (EPEC) se realizó por serotificacion utilizando a...

The present work investigated the in vitro antimicrobial activities of ethanolic, methonolic and hydroalcoholic extracts corresponding to four plants of north easter of Peru; Cassia reticulata (whole plant), Ilex guayusa Loes (leaves), Piper lineatum (leaves), y Terminalia catappa (leaves). The plants were collected in the department of Cajamarca, except Terminalia catappa (Amazonas). The antimicrobial activity was determinated by the method of agar diffusion. The used microorganisms were the bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli; and the fungals Candida albicans, Aspergillus niger and Microsporum canis. Of twelve investigated extracts, eight (67%) presented significant antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis and one (8%) against Escherichia coli. Of twelve inv...

The present work investigated the in vitro antimicrobial activities of ethanolic, methonolic and hydroalcoholic extracts corresponding to four plants of north easter of Peru; Cassia reticulata (whole plant), Ilex guayusa Loes (leaves), Piper lineatum (leaves), y Terminalia catappa (leaves). The plants were collected in the department of Cajamarca, except Terminalia catappa (Amazonas). The antimicrobial activity was determinated by the method of agar diffusion. The used microorganisms were the bacteria Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli; and the fungals Candida albicans, Aspergillus niger and Microsporum canis. Of twelve investigated extracts, eight (67%) presented significant antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis and one (8%) against Escherichia coli. Of twelve inv...

It was studied the time of permanence of Vibrio cholerae 01 in the transport media Amies, Amies with charcon and Stuart. Thus, the faecal samples of thirteen cholera patients were collected and transported in those media included Cary - Blair medium. Through the fortnight cultures of each sample, V. cholerae 01 was isolated as follow: during the firsts 30 days in Stuart medium (p=l.OO). During 45 days Cary-Blair medium (p=0.095) and during 60 days in Amies wilh charcoal medium (p=Ü.199). Results indicate that V. cholerae 01 could be transported in all the media evalualed, except in Amies medium. In those media V. chalerae 01 has recovered under its culturable and viable form during the study time.

It was studied the time of permanence of Vibrio cholerae 01 in the transport media Amies, Amies with charcon and Stuart. Thus, the faecal samples of thirteen cholera patients were collected and transported in those media included Cary - Blair medium. Through the fortnight cultures of each sample, V. cholerae 01 was isolated as follow: during the firsts 30 days in Stuart medium (p=l.OO). During 45 days Cary-Blair medium (p=0.095) and during 60 days in Amies wilh charcoal medium (p=Ü.199). Results indicate that V. cholerae 01 could be transported in all the media evalualed, except in Amies medium. In those media V. chalerae 01 has recovered under its culturable and viable form during the study time.

The objective of this paper is ta demonstrate the in vitro antimycotic activity and elucidation of sorne metabolites of the essentiaI oil of Minthostachys mollis (muña) that comes from the district of Huacrapuquio (2700m. altitude) in the province of Tarma. The essential oil of the leaves of Minthostachys mollis (muña) has been extracted by hydrodistillation method. This was subjected to Physical-Chemical analysis, Gas Chromatography (CC) to determine the chemical compasitian and then determining that fungicidal and fungistatic activity to be due to the following Monoterpenes: Pulegone, Limoneno, Menthone and also Myrcene. The antimycotic activity for Candida albicans was determined by the Agar-well diffusion assay and the method of dilution in tubes, the inhibition of growth of Trichophytun tonsurans, Trichophytun mentagophytus, Microsporun canis. For the diffusion in Agar test the di...

A study of the essential oil extracted from the leaves and flowers of the Thymus vulgris L. ''Tomillo'' by stearn distillation method was carried out. Samples were determined by physico-chemical analyses and chemical composition was obtained by GC/MS mass spectrophotometer coupled to gaschromatographer, determining the following hydrocarbonated compounds like: Thujene; 1,3,6 Octatriene,3,7-Dimethyl; Camphene; Beta Myrcene; Delta 4 Carena; Beta Selinene; Delta Cadinene; Benzene 1 methyl, 4 (l-methyl ethyl); Gama Terpinene; Trans Caryophyllene; Germacrene D.; Trans Caryophyllene; Beta Bisabolene y compuesto oxigenados como: 7 octen, 401 (CAS); l.6 octadien, 3 0l, 3,7 Dimethyl Acetate; Linalool; Isoborneol; carvacrol; Thymol; Farnesyl acetate 2.At the same time, antimicrobial activity was determined against pathogenic strains from clinical samples and from ATCC (American Typical Cultivate C...

El presente trabajo tiene por finalidad demostrar la actividad antimicótica in vitro y la elucidación de algunos de los metabolitos del aceite esencial de Minthostachys mollís (Muña) proveniente del distrito de Huacrapuquio (2700 msnm), Provincia de Tarma. El aceite esencial de las hojas de Minthostachys mollís obtenido por el método de destilación por arrastre de vapor de agua, fue sometido a análisis físico-químico y elucidación de composición química mediante cromatografía de gases (CG), se determinó los siguientes monoterpenos: Pulegona, Limoneno, Mentona y Mirceno. Mediante el método de agar en difusión, se determinó la actividad antimicótica, frente a las cepas de Candida albicans y por el método de dilución en tubo la inhibición del crecimiento fúngico de Trichophytun tonsurans, Trichophytun mentagophytus, Mícrosporun Canis. Los diámetros de la prueba de d...

A study of the essential oil extracted from the leaves and flowers of the Thymus vulgris L. ''Tomillo'' by stearn distillation method was carried out. Samples were determined by physico-chemical analyses and chemical composition was obtained by GC/MS mass spectrophotometer coupled to gaschromatographer, determining the following hydrocarbonated compounds like: Thujene; 1,3,6 Octatriene,3,7-Dimethyl; Camphene; Beta Myrcene; Delta 4 Carena; Beta Selinene; Delta Cadinene; Benzene 1 methyl, 4 (l-methyl ethyl); Gama Terpinene; Trans Caryophyllene; Germacrene D.; Trans Caryophyllene; Beta Bisabolene y compuesto oxigenados como: 7 octen, 401 (CAS); l.6 octadien, 3 0l, 3,7 Dimethyl Acetate; Linalool; Isoborneol; carvacrol; Thymol; Farnesyl acetate 2.At the same time, antimicrobial activity was determined against pathogenic strains from clinical samples and from ATCC (American Typical Cultivate C...

Tuberculosis is a public health problem in the world and Peru, aggravated by multidrug (MDR-TB) and extensively resistant (XDR-TB) forms. The objetives were to describe the resistance to tuberculosis drugs and risk factors to acquire MDR-TB in a marginal urban hospital of Lima, since 2006 to 2008. It is a reported cases studyand was undertaken in Huaycán Hospital. The data collected were acquired from the routine reports of the National Control Program and clinical history. A total of 61 cases of MDR-TB, one of XDR-TB and 7 cases of mono resistant tuberculosis were evaluated; 38 were men (55%) and 31 women (45%). The more affected age group were 15 to 34 years with 55 cases (79,6%). The more frecuent risk factors founded to acquire MDR-TB were: suspicion of failure (FS1, 34 cases, 49%) and failure of treatment one scheme (F1, 14 cases, 20%), and to have had contact with MDR-TB patients ...

Tuberculosis is a public health problem in the world and Peru, aggravated by multidrug (MDR-TB) and extensively resistant (XDR-TB) forms. The objetives were to describe the resistance to tuberculosis drugs and risk factors to acquire MDR-TB in a marginal urban hospital of Lima, since 2006 to 2008. It is a reported cases studyand was undertaken in Huaycán Hospital. The data collected were acquired from the routine reports of the National Control Program and clinical history. A total of 61 cases of MDR-TB, one of XDR-TB and 7 cases of mono resistant tuberculosis were evaluated; 38 were men (55%) and 31 women (45%). The more affected age group were 15 to 34 years with 55 cases (79,6%). The more frecuent risk factors founded to acquire MDR-TB were: suspicion of failure (FS1, 34 cases, 49%) and failure of treatment one scheme (F1, 14 cases, 20%), and to have had contact with MDR-TB patients ...

The aim of this study was to determine the antibacterial activity of essential oil Minthostachys mollis “ruyaq muña”, against Helicobacter pylori, Shigella dysenteriae, Salmonella typhi and Pseudomonas aeruginosa. The leaves of M. mollis were collected in the district of Huamanguilla (3000-3200 m.s.n.m), Huanta province, Ayacucho region. The essential oil obtained by distillation with water vapor drag. The antibacterial activity was determined by plate cultive excavation method, resulting in order of sensitivity, for S. dysenteriae 21.41 mm; H. pylori 17,07 mm; S. typhi 14.25 mm and P. aeruginosa 11.45 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for H. pylori were determined by the microplate dilution method, resulting in 2 μg/mL. For the other bacteria was determined by the dilution method, being for S. dysenteriae 4 μg/ml, S. typhi...

The aim of this study was to determine the antibacterial activity of essential oil Minthostachys mollis “ruyaq muña”, against Helicobacter pylori, Shigella dysenteriae, Salmonella typhi and Pseudomonas aeruginosa. The leaves of M. mollis were collected in the district of Huamanguilla (3000-3200 m.s.n.m), Huanta province, Ayacucho region. The essential oil obtained by distillation with water vapor drag. The antibacterial activity was determined by plate cultive excavation method, resulting in order of sensitivity, for S. dysenteriae 21.41 mm; H. pylori 17,07 mm; S. typhi 14.25 mm and P. aeruginosa 11.45 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for H. pylori were determined by the microplate dilution method, resulting in 2 μg/mL. For the other bacteria was determined by the dilution method, being for S. dysenteriae 4 μg/ml, S. typhi...

We are analyzed forty samples of raw milk commercialized at the Callao Market between January and March 2009 using presumptive method for β-lactamics and tetracycline antibiotic residues from IDEX laboratories, to comply with the limits of sensitivity of residues of the FDA. We found 40% of samples tested positive for β-lactamic residues in raw milk. There were no positive outcame to analyze tetracyclines. The results suggest that we should establish policies for legislation, regulation and training and that the NTP 202.001.2003 should be reviewed. The results suggested that should be established policies for legislation, regulation and training, and the NTP 202. 00102003 should be reviewed.

We are analyzed forty samples of raw milk commercialized at the Callao Market between January and March 2009 using presumptive method for β-lactamics and tetracycline antibiotic residues from IDEX laboratories, to comply with the limits of sensitivity of residues of the FDA. We found 40% of samples tested positive for β-lactamic residues in raw milk. There were no positive outcame to analyze tetracyclines. The results suggest that we should establish policies for legislation, regulation and training and that the NTP 202.001.2003 should be reviewed. The results suggested that should be established policies for legislation, regulation and training, and the NTP 202. 00102003 should be reviewed.

There was studied the specie Tagetes pusilla "anisillo" " saw anise's ", which is a medicine original Asteraceae of use medical of our country, whichgrows abundant in Ayacucho's department, Tambillo's locality, between 2800 to 3000 msnm and which according to the information provided by the etnobotanic, popular medicine, anthropologic information, they indicate its uses as infusions of the air part and its essential oil, for to relieve affections of the digestive tract and for the lactic secretion, besides diuretic. In the present investigation the types detected and identified of secondary metabolits predominant in the extract etanólico of leaves of anisillo, that were fenolics compounds, flavonoids and alkaloids. The antibacterial comparatíve activity decided between the essential oil and the etanólico extract of anisillo, thinking that both showed an antibacterial significant activ...

There was studied the specie Tagetes pusilla "anisillo" " saw anise's ", which is a medicine original Asteraceae of use medical of our country, whichgrows abundant in Ayacucho's department, Tambillo's locality, between 2800 to 3000 msnm and which according to the information provided by the etnobotanic, popular medicine, anthropologic information, they indicate its uses as infusions of the air part and its essential oil, for to relieve affections of the digestive tract and for the lactic secretion, besides diuretic. In the present investigation the types detected and identified of secondary metabolits predominant in the extract etanólico of leaves of anisillo, that were fenolics compounds, flavonoids and alkaloids. The antibacterial comparatíve activity decided between the essential oil and the etanólico extract of anisillo, thinking that both showed an antibacterial significant activ...