Piper aduncum es un arbusto o arbolillo distribuido a lo largo de América en el que se han identificado numerosos compuestos biológicamente activos. Diferentes combinaciones de reguladores de crecimiento fueron ensayadas para la propagación clonal, la inducción y mantenimiento de callos y el establecimiento de suspensiones celulares. Los resultados mostraron una significativa mayor elongación del brote en el tratamiento con medio de cultivo B5 suplementado con AIA 0,02 mg/L y AG3 0,02 mg/L en comparación con otros tratamientos, la inducción de callos en varios tratamientos con BAP o ANA-BAP y el establecimiento de suspensiones celulares en varias combinaciones de auxinas (AIA, ANA o 2,4-D) - citocininas (BAP o KIN). A partir de los callos formados se establecieron suspensiones celulares y estudió la acumulación de metabolitos secundarios. En ese sentido, en suspensiones celulare...

Cucurbitaceae species constitute a family with a wide worldwide distribution and great nutritional and medicinal importance. The Cucurbita genus is the most important of the family with valuable species such as C. maxima and C. moschata. However, the large-scale production of sex specific plants using the conventional propagation methods has various limitations. The tissue culture such as micropropagation can help overcome these limitations. The aim of this study was to establish a protocol for ex vitro and in vitro clonal propagation of two varieties of Cucurbita moschata: ‘loche’, a variety of commercial vegetative propagation and endemic in Lambayeque, and ‘chuyán’ another variety of very restricted cultivation in some Andean regions of the north of the Peru. Likewise, the in vitro propagation of ‘chisguín’ (C. ecuadorensis), a threatened endemic wild species from the co...

Loxopterygium huasango Spruce ex Engl., of the Anacardiaceae family, classified critically endangered, is a forest tree with considerable value in the Seasonally Dry Tropical Forest (SDTF) in the northwest of Peru and south of Ecuador. The aim of the study was callus induction, propagation and germplasm conservation using in vitro tissue culture techniques. In vitro seed germination (90%) was obtained on Murashige and Skoog (MS) medium supplemented with 5.0 mg/L-1 GA3. The cotyledonal nodes of seedlings were used as explants to initiate the growth of the apical shoots, in culture medium with the IAA-GA3 interactions, without observing growth of the axillary buds. The highest rate of friable callus induction (100%) was recorded on MS medium containing 0.1 to 5.0 mg/L-1 2,4-D in cotyledons and hypocotyls explants after 30 to 45 days of culture. The profuse roots formation was the only morp...

Plant biotechnology is one of the most important tools presently available and methods to genetically manipulate plants to exhibit greater productivity, tolerance to physical and chemical stress, and resistance to pests and diseases have been developed in recent decades. In vitro plant tissue culture has contributed significantly to this purpose. The objective of this study is to apply various techniques developed by in vitro tissue culture in seed germination, meristem culture for virus elimination, micropropagation, conservation, international transfer of germplasm, and induction of various morphogenic processes that lead to the genetic variability known as somaclonal variation. The application of this set of biotechnological techniques, in crops such as cassava, sweet potato, potato, native forest species as Cedrela odorata, Ficus spp. Loxopterygium huasango, and others, sugar cane, r...

Potato plants are often subjected to heat stress during growth and development, resulting in loss of yield and quality. However, the possibility exists that commercially important genotypes show tolerance to heat stress. In this study, we aim to investigate the morphological and physiological effects of moderate conditions of heat stress on tuberization in nine potato genotypes, and how it affects propagation using an ex vitro and in vitro system. The plant material came from the In Vitro Germplasm Bank of the International Potato Center (CIP). The incubation temperatures of the cultures in in vitro and ex vitro conditions were the ambient in the laboratory and greenhouse, respectively. In in vitro system a randomized complete block design was employed in nine genotypes, two treatments: multiplication culture medium and the same culture medium supplemented with 8.0% sucrose and 500 mg/L-...

The Amazon rainforest is one of the most biodiverse and at the same time most threatened ecosystems in the biosphere. Its different habitats provide a unique floristic pattern. Therefore, we aimed to identify the floristic arboreal composition in an area of the Amazon rainforest and determine the local patterns of diversity in its different habitats. This study was conducted around the Tambopata Research Center, Madre de Dios, Peru. To achieve our goals, we established 20 (50 × 30 m) plots distributed equally in four habitats, these being: Mauritia Palm Swamp, Floodplain Forest, Successional Floodplain Forest, and Tierra Firme Forest. In each plot, we collected all individuals with a diameter at a breast height ≥ of 10 cm. Subsequently, the individuals were identified and classified by cluster and diversity analysis. We found 57 families, 173 genera, 300 species, and 1958 individuals....

The Amazon rainforest is one of the most biodiverse and at the same time most threatened ecosystems in the biosphere. Its different habitats provide a unique floristic pattern. Therefore, we aimed to identify the floristic arboreal composition in an area of the Amazon rainforest and determine the local patterns of diversity in its different habitats. This study was conducted around the Tambopata Research Center, Madre de Dios, Peru. To achieve our goals, we established 20 (50 × 30 m) plots distributed equally in four habitats, these being: Mauritia Palm Swamp, Floodplain Forest, Successional Floodplain Forest, and Tierra Firme Forest. In each plot, we collected all individuals with a diameter at a breast height ≥ of 10 cm. Subsequently, the individuals were identified and classified by cluster and diversity analysis. We found 57 families, 173 genera, 300 species, and 1958 individuals....

Con el objetivo de contribuir al conocimiento de los ecosistemas naturales del país, se determinó las características edáficas y la composición florística del bosque estacionalmente seco (La Menta, 04º 43' 55” LS y 80º 04' 55” LO, provincia de Piura y Timbes, 04º 44' 58” LS y 80º 03' 33” LO, provincia de Ayabaca), ambos en la región Piura, Perú. El área de estudio abarcó una extensión aproximada de 6 000 hectáreas en un rango altitudinal entre 350 m a 1 350 m. En cada bosque se muestrearon 5 transectos de 10 x 50 m a intervalos de 20 m, sumando un total de 2 500 m2. El análisis de suelo reveló niveles bajos de materia orgánica, nitrógeno y potasio y medios de fósforo asimilable. Estos suelos han sido clasificados en el orden Entisoles y suborden Orthents. En el bosque La Menta se registraron 18 especies, entre árboles y arbustos con diámetro a la al...