Plant biotechnology is one of the most important tools presently available and methods to genetically manipulate plants to exhibit greater productivity, tolerance to physical and chemical stress, and resistance to pests and diseases have been developed in recent decades. In vitro plant tissue culture has contributed significantly to this purpose. The objective of this study is to apply various techniques developed by in vitro tissue culture in seed germination, meristem culture for virus elimination, micropropagation, conservation, international transfer of germplasm, and induction of various morphogenic processes that lead to the genetic variability known as somaclonal variation. The application of this set of biotechnological techniques, in crops such as cassava, sweet potato, potato, native forest species as Cedrela odorata, Ficus spp. Loxopterygium huasango, and others, sugar cane, r...

Cucurbitaceae species constitute a family with a wide worldwide distribution and great nutritional and medicinal importance. The Cucurbita genus is the most important of the family with valuable species such as C. maxima and C. moschata. However, the large-scale production of sex specific plants using the conventional propagation methods has various limitations. The tissue culture such as micropropagation can help overcome these limitations. The aim of this study was to establish a protocol for ex vitro and in vitro clonal propagation of two varieties of Cucurbita moschata: ‘loche’, a variety of commercial vegetative propagation and endemic in Lambayeque, and ‘chuyán’ another variety of very restricted cultivation in some Andean regions of the north of the Peru. Likewise, the in vitro propagation of ‘chisguín’ (C. ecuadorensis), a threatened endemic wild species from the co...

Potato plants are often subjected to heat stress during growth and development, resulting in loss of yield and quality. However, the possibility exists that commercially important genotypes show tolerance to heat stress. In this study, we aim to investigate the morphological and physiological effects of moderate conditions of heat stress on tuberization in nine potato genotypes, and how it affects propagation using an ex vitro and in vitro system. The plant material came from the In Vitro Germplasm Bank of the International Potato Center (CIP). The incubation temperatures of the cultures in in vitro and ex vitro conditions were the ambient in the laboratory and greenhouse, respectively. In in vitro system a randomized complete block design was employed in nine genotypes, two treatments: multiplication culture medium and the same culture medium supplemented with 8.0% sucrose and 500 mg/L-...

Con el objetivo de contribuir al conocimiento de los ecosistemas naturales del país, se determinó las características edáficas y la composición florística del bosque estacionalmente seco (La Menta, 04º 43' 55” LS y 80º 04' 55” LO, provincia de Piura y Timbes, 04º 44' 58” LS y 80º 03' 33” LO, provincia de Ayabaca), ambos en la región Piura, Perú. El área de estudio abarcó una extensión aproximada de 6 000 hectáreas en un rango altitudinal entre 350 m a 1 350 m. En cada bosque se muestrearon 5 transectos de 10 x 50 m a intervalos de 20 m, sumando un total de 2 500 m2. El análisis de suelo reveló niveles bajos de materia orgánica, nitrógeno y potasio y medios de fósforo asimilable. Estos suelos han sido clasificados en el orden Entisoles y suborden Orthents. En el bosque La Menta se registraron 18 especies, entre árboles y arbustos con diámetro a la al...