Fructooligosaccharides (FOS) are fructose polymers, linked by β-glucosidic bonds (2-1), considered low caloric food sweeteners with prebiotic function and can be obtained from sucrose by biosynthesis. The fruits of the carob tree (Prosopis pallida) contain mainly carbohydrates where sucrose stands out. For this reason, the production of FOS from carob extract was optimized using a pectinase from A. aculeatus. The response surface methodology using was applied to optimize the parameters that affected production: time, temperature and carob extract; then they were verified experimentally. The biosynthesis process of FOS was carried out with carob extract at the concentrations of 20, 35 and 50 ° Bx, Pectinase at 10, 35 and 61mg, at 45, 55 and 65 °C for 0,5, 9,5 and 18 h, with stirring at 150 rpm The analysis methods were glucose oxidase and AOAC 999.03, for glucose and FOS respectively a...

El objeto del presente trabajo fue el incremento de fructooligosacáridos en el extracto de yacón (Smallantus sonchifolius) a partir de azúcares fermentables usando fructosiltransfertasas. Para ello, se buscó optimizar los parámetros críticos involucrados en la biosíntesis de fructooligosacáridos como pH, tiempo, temperatura y concentración de extracto de yacón mediante la metodología de respuesta (RSM, Response Surface Method), los cuales se verificaron experimentalmente con el fin de incrementar la concentración de fructooligosacáridos. El proceso de transfructosilación se llevó a cabo en tubos de ensayo, en donde se adiciono diferentes concentraciones de extracto de yacón 20, 40 y 60 °Bx, enzima celulasa 4,07; 103,89 y 203,70 mg, y temperaturas de 45, 55 y 65 °C durante 12 h en un agitador de tipo vaivén a 150 rpm tomándose muestras a 0,17 h; 6,15 h y 12 h. Para la ...

Pretende obtener fructooligosacáridos (FOS) por síntesis enzimática a partir de los carbohidratos presentes en los frutos de algarrobo (Prosopis pallida). Para ello, primero se evaluaron las enzimas comerciales inulinasa, invertasa y pectinasa con sacarosa al 30 % a 55 °C, 150 rpm y 60 min. Por otro lado, de los frutos de algarrobo se preparó la harina, realizó el análisis proximal, extrajeron y concentraron los carbohidratos hasta 20, 35 y 50 °Bx, para luego ser almacenados a 8 °C. Los parámetros de optimización considerados en la metodología de superficie de respuesta fueron: tiempo, temperatura, concentraciones de enzima y extracto acuoso de algarrobo; posteriormente estos parámetros se verificaron experimentalmente. La biosíntesis de FOS fue realizado mezclando extracto de algarrobo a las concentraciones de 20, 35 y 50 °Bx y pectinasa de Aspergillus aculeatus a 10, 35 ...

Objective: Paraoxonase 1 (PON1), an esterase associated with high-density lipoproteins (HDL), has several polymorphisms: the PON1 Q192R polymorphism (coding region) is responsible for variations in the paraoxonase activity of the enzyme. The PON1 192R allele is considered a risk factor for developing cardiometabolic disorders in some populations. The aim of the present study is to determine the distribution of the PON1 Q192R polymorphism and its association with the lipid profile and APO A1 in an Andean population. Materials and methods: A descriptive, cross-sectional study involving 79 healthy adults (26 males and 53 females), residents of the district of Junín at 4105 m.a.s.l. The PCR/RFLP technique was used for the analysis of the PON1 Q192R polymorphism. The serum values of the lipid profile and APO A1, and the paraoxonase and arylesterase activities of the enzyme were determined. R...

Objectives: To determine the serum levels of sCD36, a lipid metabolism-related molecule, in high-altitude and sea-level populations, and to establish the association of this parameter with cardiometabolic risk factors. Materials and methods: The study population consisted of 45 people from Carhuamayo (4100 masl) and 40 people from Mala (30 masl). Weight, height and blood pressure were measured. Hemoglobin was determined in whole blood, and glucose, lipid profile and sCD36 in serum. Results: It has been found that hemoglobin levels in the population of Carhuamayo were significantly higher, while weight, BMI and glucose level were significantly lower than those in the population of Mala. There was no significant difference between serum levels of sCD36 in both populations. A significant difference was observed between sCD36 mean serum levels of both populations based on the BMI, and a sign...