The growth probability of Alicyclobacillus acidoterrestris CRA 7152 in orange juice was studied in different product conditions. The microorganism response was monitored until 47 days of storage in different conditions of pH (3 to 5.8), soluble solids (11 to 19°Brix), temperature (20 to 54°C) and nisin concentration (0 to 70 IU/ml). Growth/no growth data were modeled by the polynomial logistics regression model. The concordance of the obtained model was 96.3% indicating good fitting of observed data. The results showed fast growth in the conditions 0 IU nisin/ml juice, pH 4.4 and 15oBrix, at 35oC. For 70 IU nisin/ml there was no growth, pH 4.4, at 37oC, up to 47 days of storage. Simple juices (11°Brix) with pHs between 3.5 to 3.7 can keep stable and withstand abuse temperatures up to 36°C, since added in 70IU nisin/ml, extending its shelf life. With 0.05 growth probability and using ...

The growth probability of Alicyclobacillus acidoterrestris CRA 7152 in orange juice was studied in different product conditions. The microorganism response was monitored until 47 days of storage in different conditions of pH (3 to 5.8), soluble solids (11 to 19°Brix), temperature (20 to 54°C) and nisin concentration (0 to 70 IU/ml). Growth/no growth data were modeled by the polynomial logistics regression model. The concordance of the obtained model was 96.3% indicating good fitting of observed data. The results showed fast growth in the conditions 0 IU nisin/ml juice, pH 4.4 and 15oBrix, at 35oC. For 70 IU nisin/ml there was no growth, pH 4.4, at 37oC, up to 47 days of storage. Simple juices (11°Brix) with pHs between 3.5 to 3.7 can keep stable and withstand abuse temperatures up to 36°C, since added in 70IU nisin/ml, extending its shelf life. With 0.05 growth probability and using ...

Descubrir cómo el estrés afecta la productividad del personal de enfermería que trabaja en un servicio de urgencias de una clínica en San Isidro, Lima, en 2024 fue el motor de esta investigación. El estudio empleó una metodología cuantitativa e hipotético-deductiva, de naturaleza transversal y con un enfoque correlacional. Se seleccionaron treinta profesionales de enfermería de forma aleatoria de la población mediante un muestreo censal. Una prueba de fiabilidad con un alfa de Cronbach superior a 0,88 indicó que se administraron los cuestionarios validados utilizados para recopilar datos sobre estrés laboral (considerando cansancio emocional, despersonalización y realización personal) y desempeño laboral. Se utilizó el programa SPSS versión 27 para procesar los datos, que incluyó estadística descriptiva e inferencial. Se empleó el coeficiente de correlación de Spearm...

En la gestión de la seguridad y salud en obras eléctricas de baja tensión, la situación problemática radica en los riesgos significativos asociados con el contacto directo con partes activas, que pueden causar graves daños como fulguración y quemaduras. A pesar de la existencia de normativas como la Ley N° 29783 y el Decreto Supremo N° 003-2013-TR en Perú, la implementación efectiva de estas normativas sigue siendo un desafío, especialmente en contextos donde los trabajadores no están adecuadamente capacitados. La estrategia metodológica adoptada incluye técnicas mixtas de recolección de datos: encuestas a trabajadores y supervisores, entrevistas semiestructuradas, observación directa de prácticas de trabajo y revisión documental de registros de incidentes y programas de capacitación. La muestra se seleccionó mediante un muestreo estratificado, asegurando la represen...

This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, α-lactalbumin and β-lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion Exchange chromatography and size-exclusion chromatography. The reverse- phase liquid chromatography led to a better separation of whey proteins than size-exclusion chromatography and anion exchange chromatography, this method offered an excellent separation for whey proteins and presented a short time of analysis (33 min).

This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, α-lactalbumin and β-lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion Exchange chromatography and size-exclusion chromatography. The reverse- phase liquid chromatography led to a better separation of whey proteins than size-exclusion chromatography and anion exchange chromatography, this method offered an excellent separation for whey proteins and presented a short time of analysis (33 min).