ABSTRACT The present research was carried out in the adjacent mangroves to the Faculty of Fishing Engineering of the National University of Tumbes (3°30'21,43" S and 80°23'48,36" W), from January 25th of 2012 to January 26th of 2013, the general objective was to determine the effect of the sowing density on the increment in length and weight, as well as the survival of Anadara tuberculosa sowed in its natural environment, the swamp, to three densities with seeds obtained in laboratory. To achieve the purpose, corrals of 2m x 1m where settled, there were three treatments t1=20, t0=40, and t2=60 (ind./m-2), during twelve months. During the investigation the total length of the valve was measured (lt), height of the valve (h), thickness of the valve, all in millimetrer and the total weight (pt) in grams. During the first five months the increment in length as well as in weight was smaller...

No description

No description

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...