The study aimed to describe the histological features of Peyer’s patches in jejunum and ileum of 33 newborn alpacas from 0 to 45 days of age. Sections of jejunum and ileum were collected and fixed in 10% buffered formaldehyde. Standard histological slides stained with hematoxylin-eosin were obtained and poly-L-lysine coated slides were used for detection of T cells by immunohistochemistry using monoclonal antibodies against CD-8 marker aided by Histostain development system. Histologically, newborn alpacas showed organized and well defined intestinal mucosa-associated lymphoid tissue in both, jejunum and ileum. In animals older than 14 days of age, jejunal Peyer’s patches develop showing regions of active cells and other areas of undifferentiated lymphoid cells. It was also found a very active and positive reaction to CD8 marker on lymphocytes grouped in follicles and on Peyer’s pa...

The study aimed to describe the histological features of Peyer’s patches in jejunum and ileum of 33 newborn alpacas from 0 to 45 days of age. Sections of jejunum and ileum were collected and fixed in 10% buffered formaldehyde. Standard histological slides stained with hematoxylin-eosin were obtained and poly-L-lysine coated slides were used for detection of T cells by immunohistochemistry using monoclonal antibodies against CD-8 marker aided by Histostain development system. Histologically, newborn alpacas showed organized and well defined intestinal mucosa-associated lymphoid tissue in both, jejunum and ileum. In animals older than 14 days of age, jejunal Peyer’s patches develop showing regions of active cells and other areas of undifferentiated lymphoid cells. It was also found a very active and positive reaction to CD8 marker on lymphocytes grouped in follicles and on Peyer’s pa...

The aim of this study was to determine by real-time RT-PCR the presence and relative levels of tumor necrosis factor alpha (TNF-a) and interleukin 1 alpha (IL-1a) mRNA expression in intestinal mucosa of healthy and with enteropathy young alpacas (Vicugna pacos) from Cusco, Peru. A total of 71 samples of jejunum from 0 to 45 days old healthy (n=38) and diseased (n=33) alpacas were processed. Total RNA was extracted from samples and to the synthesis of DNA complementary strand (cDNA) by reverse transcription (RT) was done. Subsequently, conventional PCR and real time RT-PCR was conducted. The results showed that 98.6% (70/71) of the samples amplified a specific product of 251 pb for TNF-a and 100% (71/71) of the samples amplified a specific product of 172 pb for IL-1a, evidenced in the agarose gel during electrophoresis. Analysis of dissociation curves determined that: a) the products ampl...

The aim of this study was to determine by real-time RT-PCR the presence and relative levels of tumor necrosis factor alpha (TNF-a) and interleukin 1 alpha (IL-1a) mRNA expression in intestinal mucosa of healthy and with enteropathy young alpacas (Vicugna pacos) from Cusco, Peru. A total of 71 samples of jejunum from 0 to 45 days old healthy (n=38) and diseased (n=33) alpacas were processed. Total RNA was extracted from samples and to the synthesis of DNA complementary strand (cDNA) by reverse transcription (RT) was done. Subsequently, conventional PCR and real time RT-PCR was conducted. The results showed that 98.6% (70/71) of the samples amplified a specific product of 251 pb for TNF-a and 100% (71/71) of the samples amplified a specific product of 172 pb for IL-1a, evidenced in the agarose gel during electrophoresis. Analysis of dissociation curves determined that: a) the products ampl...