“In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25...

“In the present investigation, an ethanolic fraction (EF) of Lepechinia meyenii (salvia) was prepared and fractionated by gradient column chromatography, and the main secondary metabolites present in the EF were identified by HPLC-MS. Silver nanoparticles (AgNPs) were synthesized and conjugated with the EF of Lepechinia meyenii (salvia). The AgNPs synthesis was optimized using Plackett-Burman design and response surface methodology (RSM), considering the following independent variables: stirring speed, synthesis pH, synthesis time, synthesis temperature and EF volume. The AgNPs synthesized under the optimized conditions were characterized by UV visible spectroscopy (UV-VIS), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM). The antibacterial activity of the AgNPs against Staphylococcus aureus (ATCC® 25...

Para el presente trabajo se propuso optimizar la biosíntesis de nanopartículas de plata NPsAg por Síntesis Verde, y obtener un modelo matemático cuadrático valido, que nos represente los datos experimentales obtenidos, usando la Metodología Superficie de Respuesta – MSR. La biosíntesis de NPsAg, fue realizada usando la Fracción Flavonoide (FF), obtenida a partir de la planta medicinal peruana Lepechinia meyenii – “Salvia”. Para la obtención de las condiciones optimizadas de la biosíntesis de NPsAg, se tuvo como objetivo principal diseñar un modelo estadístico polinómico, aplicando la MSR. Por consiguiente, en la etapa I “Screening”, se empleó el Diseño Placket y Burman (P&B); para discriminar las variables no significativas y obtener un modelo estadístico multifactorial de 1er orden; en la etapa II “Escalamiento” se localizó la región óptima del proceso...