La presente investigación tuvo como objetivo evaluar la capacidad de biodegradación de hidrocarburos del petróleo de cepas bacterianas aisladas en el manglar de la localidad de Puerto Pizarro, en el distrito, provincia y región Tumbes. Se aislaron 12 cepas de bacterias para evaluar tal capacidad. A cada cepa se le extrajo el ADN y se amplificó por PCR con iniciadores dirigidos al gen 16S ARNr y a genes específicos de enzimas oxigenas de hidrocarburos. Además se realizaron pruebas en cinco de las cepas aisladas para determinar la biodegradación de petróleo tanto a nivel de laboratorio como de campo. A nivel de laboratorio, las cepas fueron sometidas a crecimiento en medio mineral Bushnell Hass suplementado con petróleo como única fuente de carbono. A nivel de campo se realizó un ensayo durante 30 días adicionando de forma individual las cepas bacterianas sobre un suelo de man...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...

The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible ...