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OBJECTIVE: To determine Aeromonas importance as diarrhea agent in children less than 5 year- old and compare its frequency with other enteropathogens. MATERIAL AND METHODS: Between 1998 and 1999 285 fecal samples were taken from children with acute diarrhea in 5 hospitals, which were then sent to “Daniel A. Carrión” Institute of Tropical Medicine in Cary Blair’s transportation medium for processing by Aerokey II method. RESULTS: Aeromonas was the most frequent bacteria isolated among enteropathogens, mainly in children less than 2-year old and in summer, and Aeromonas caviae was the most frequent species. Aeromonas was mainly isolated in agar TCBS, employing O/129 disks to differentiate it from Vibrio cholerae. All Aeromonas were sensitive in vitro to furazolidone and neomycin. CONCLUSIONS: Aeromonas was the main etiological agent of aquous acute diarrhea in children less than 5 year...
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Objective: To identify Malassezia species in healthy skin seborrhea areas in Lima inhabitants. Design: Cross-sectional study. Setting: Daniel Alcides Carrion Tropical Medicine Institute, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: Asymptomatic persons. Interventions: Skin samples were collected from 129 asymptomatic residents of several districts of Lima city using Mariat and Adan-Campos’ technique. Malassezia isolation was performed in modified Dixon medium and incubated at 32° C for 7 days. Colonies were identified by macro and micro morphological characteristics and typing was determined by biochemical and physiological properties using Guillot’s technique. Main outcome measures: Malassezia species, participants’ gender, age and anatomical region. Results: Malassezia spp was isolated in 43.4% of the residents, 49.2% in men and 37.5% in women. From variou...