The aim of the present study was to evaluate the in vitro efficacy of Cordyceps bassiana for the control of the adult stage of Rhipicephalus microplus. The adult immersion test in a solution containing the BbF2011 strain of C. bassiana at the concentrations of 1x104, 1x106 and 1x108 conidia/ml (30 ticks per treatment) was evaluated. Treated ticks at a concentration of 1x108 conidia/ml reached 100% mortality at 15 days post-treatment (PT), while ticks at concentrations of 1x106 y 1x104 conidia/ml reached it at 18 and 21 days PT, respectively. Tick mortality was directly related to the fungi concentration. Moreover, ticks at the concentration 1x108 conidia/ml showed the lowest oviposition and larva hatching as compared to 1x106 and 1x104 conidia/ml concentrations (p<0.05). The effectiveness shown by C. bassiana on R. microplus opens the option to a new biological control method.

En el presente estudio se evaluó la eficacia in vitro de Cordyceps bassiana en el control biológico de la fase adulta de Rhipicephalus microplus. Se aplicó la técnica de inmersión de garrapatas adultas en una solución con la cepa BbF2011 de C. bassiana en concentraciones de 1x104, 1x106 y 1x108 conidias/ml (30 garrapatas por tratamiento). Las garrapatas tratadas a una concentración de 1x108 conidias/ml alcanzaron el 100% de mortalidad al día 15 postratamiento (PT), mientras que las concentraciones 1x106 y 1x104 conidias/ml lo obtuvieron en los días 18 y 21 PT, respectivamente (p<0.05). La mortalidad fue directamente proporcional a la concentración de C. bassiana. Asimismo, las garrapatas en la concentración de 1x108 conidias/ml presentaron una menor postura de huevos y porcentaje de eclosión de larvas que los tratamientos con 1x106 y 1x104 conidias/ml (p<0.05). La efect...

The aim of the present study was to determine the presence of antibodies against Neospora caninum in a dairy herd in the municipality of Toca, Colombia. Blood samples (n=100) were collected from females between 1 and 9 years of age. The animals were selected through simple random method of prospective cross-cut. The serum samples were processed using a commercial indirect ELISA kit. The prevalence of antibodies anti-N. caninum was 64%. The association between positive animals with history of abortion and parity was statistically significant (p<0.05), while the association with age, reproductive status and repeat breeding was not significant.

El presente estudio tuvo como objetivo determinar la presencia de anticuerpos contra Neospora caninum en un hato lechero del municipio de Toca, Colombia. Se tomaron 100 muestras de sangre de bovinos de 1 a 9 años de edad, seleccionados mediante un método aleatorio simple de corte transversal prospectivo. Las muestras de suero fueron procesadas mediante un kit comercial de ELISA indirecta. La seroprevalencia de anticuerpos anti-N. caninum fue de 64%. La asociación entre animales positivos con historia de abortos y número de partos fue estadísticamente significativa (p<0.05), en tanto que la asociación con la edad, estado reproductivo y repetición de celos no fue significativa.

The aim of this study was to determine the prevalence of hepatic dystomatosis in cattle slaughtered in the Sogamoso slaughterhouse (Boyacá, Colombia), and to define the most effective diagnostic technique to establish the presence of the parasite. A sample size of 343 individuals was established considering the monthly slaughter rate of the plant. Stool, blood and bile samples were taken, and adult parasites were collected from the bile duct. The modified Ritchie technique was used in the coproparasitological examination, the bile content was analyzed by sedimentation and a standardized in-house ELISA was implemented for serological diagnosis from adult Fasciola hepatica parasites. The results showed that 29.7% (102/343) of the individuals sampled were positive for the presence of F. hepatica in at least one of the techniques used. The post mortem evaluation presented the highest value ...