The aim of the present study was to evaluate the in vitro efficacy of Cordyceps bassiana for the control of the adult stage of Rhipicephalus microplus. The adult immersion test in a solution containing the BbF2011 strain of C. bassiana at the concentrations of 1x104, 1x106 and 1x108 conidia/ml (30 ticks per treatment) was evaluated. Treated ticks at a concentration of 1x108 conidia/ml reached 100% mortality at 15 days post-treatment (PT), while ticks at concentrations of 1x106 y 1x104 conidia/ml reached it at 18 and 21 days PT, respectively. Tick mortality was directly related to the fungi concentration. Moreover, ticks at the concentration 1x108 conidia/ml showed the lowest oviposition and larva hatching as compared to 1x106 and 1x104 conidia/ml concentrations (p<0.05). The effectiveness shown by C. bassiana on R. microplus opens the option to a new biological control method.

En el presente estudio se evaluó la eficacia in vitro de Cordyceps bassiana en el control biológico de la fase adulta de Rhipicephalus microplus. Se aplicó la técnica de inmersión de garrapatas adultas en una solución con la cepa BbF2011 de C. bassiana en concentraciones de 1x104, 1x106 y 1x108 conidias/ml (30 garrapatas por tratamiento). Las garrapatas tratadas a una concentración de 1x108 conidias/ml alcanzaron el 100% de mortalidad al día 15 postratamiento (PT), mientras que las concentraciones 1x106 y 1x104 conidias/ml lo obtuvieron en los días 18 y 21 PT, respectivamente (p<0.05). La mortalidad fue directamente proporcional a la concentración de C. bassiana. Asimismo, las garrapatas en la concentración de 1x108 conidias/ml presentaron una menor postura de huevos y porcentaje de eclosión de larvas que los tratamientos con 1x106 y 1x104 conidias/ml (p<0.05). La efect...

A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection...

A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection...