The aim if this study was to determine the prevalence of Fasciola hepatica in cattle at the slaughterhouse of the municipality of Une, Cundinamarca, Colombia. Sample size was calculated based on the slaughter rate of 2013 plus 6% increase (n=142). Adult parasites were collected from the biliary duct. Bile and faecal material were also collected. The modified Ritchie-Frick technique was used to analyse faecal samples. The prevalence of F. hepatica was 39.4% based on eggs in the bile, 32.4% based on the presence of adult parasites in biliary ducts and 15.5% based on the presence of eggs in faecal samples. Positive animals for presence of adult parasites plus eggs in bile and faecal samples was 15.5%.

El objetivo del presente estudio fue determinar la prevalencia de Fasciola hepatica en bovinos sacrificados en la planta de beneficio del municipio de Une, Cundinamarca, Colombia. El tamaño muestral fue calculado con base a la tasa de sacrificio de 2013 con 6% de incremento (n=142). Durante el eviscerado se recolectaron distomas adultos del ducto biliar, así como muestras del contenido biliar y materia fecal. En el examen coproparasitológico se empleó la técnica de Ritchie-Frick modificada. La prevalencia de F. hepatica fue 39.4% por presencia de huevos en el contenido biliar, 32.4% por presencia de parásitos adultos en los ductos biliares y 15.5% por presencia de huevos en materia fecal. El valor porcentual de animales positivos por presencia de estadio adulto, así como huevos en bilis y materia fecal fue de 15.5%.

The aim of the present study was to evaluate the in vitro efficacy of Cordyceps bassiana for the control of the adult stage of Rhipicephalus microplus. The adult immersion test in a solution containing the BbF2011 strain of C. bassiana at the concentrations of 1x104, 1x106 and 1x108 conidia/ml (30 ticks per treatment) was evaluated. Treated ticks at a concentration of 1x108 conidia/ml reached 100% mortality at 15 days post-treatment (PT), while ticks at concentrations of 1x106 y 1x104 conidia/ml reached it at 18 and 21 days PT, respectively. Tick mortality was directly related to the fungi concentration. Moreover, ticks at the concentration 1x108 conidia/ml showed the lowest oviposition and larva hatching as compared to 1x106 and 1x104 conidia/ml concentrations (p<0.05). The effectiveness shown by C. bassiana on R. microplus opens the option to a new biological control method.

En el presente estudio se evaluó la eficacia in vitro de Cordyceps bassiana en el control biológico de la fase adulta de Rhipicephalus microplus. Se aplicó la técnica de inmersión de garrapatas adultas en una solución con la cepa BbF2011 de C. bassiana en concentraciones de 1x104, 1x106 y 1x108 conidias/ml (30 garrapatas por tratamiento). Las garrapatas tratadas a una concentración de 1x108 conidias/ml alcanzaron el 100% de mortalidad al día 15 postratamiento (PT), mientras que las concentraciones 1x106 y 1x104 conidias/ml lo obtuvieron en los días 18 y 21 PT, respectivamente (p<0.05). La mortalidad fue directamente proporcional a la concentración de C. bassiana. Asimismo, las garrapatas en la concentración de 1x108 conidias/ml presentaron una menor postura de huevos y porcentaje de eclosión de larvas que los tratamientos con 1x106 y 1x104 conidias/ml (p<0.05). La efect...

The aim of this study was to determine the prevalence of hepatic dystomatosis in cattle slaughtered in the Sogamoso slaughterhouse (Boyacá, Colombia), and to define the most effective diagnostic technique to establish the presence of the parasite. A sample size of 343 individuals was established considering the monthly slaughter rate of the plant. Stool, blood and bile samples were taken, and adult parasites were collected from the bile duct. The modified Ritchie technique was used in the coproparasitological examination, the bile content was analyzed by sedimentation and a standardized in-house ELISA was implemented for serological diagnosis from adult Fasciola hepatica parasites. The results showed that 29.7% (102/343) of the individuals sampled were positive for the presence of F. hepatica in at least one of the techniques used. The post mortem evaluation presented the highest value ...