We have determined antiaxidant enzyme activities of: Superoxide Dismutase (SOD), Glutatione Peroxidase (GPx) and Catalase (CAT); levels of malondialdehyde (MDA) and hydroperoxids as lipoperoxidation indicators, and phospholilpase activity as mediator of the lipoperoxidation in cerebral tissue of guinea pigs born and bred in Cerro de Pasco at 4 300 m (ALT), with respect to guinea pigs at sea level (NM), in order to detect real differences between both groups. With respect to activity of antioxidant enzymes, we have not found significant differences between both groups. In the study group (ALT) we have found significant differences in the activity of phospholipase and level of hydroperoxides (p < 0.01 Y P < 0.001. respectively) as compared to sea level group. Also, we have found that level of MDA is significantly less in the study group (p < 0.01).Thus, observed data would lead us...

We have determined antiaxidant enzyme activities of: Superoxide Dismutase (SOD), Glutatione Peroxidase (GPx) and Catalase (CAT); levels of malondialdehyde (MDA) and hydroperoxids as lipoperoxidation indicators, and phospholilpase activity as mediator of the lipoperoxidation in cerebral tissue of guinea pigs born and bred in Cerro de Pasco at 4 300 m (ALT), with respect to guinea pigs at sea level (NM), in order to detect real differences between both groups. With respect to activity of antioxidant enzymes, we have not found significant differences between both groups. In the study group (ALT) we have found significant differences in the activity of phospholipase and level of hydroperoxides (p < 0.01 Y P < 0.001. respectively) as compared to sea level group. Also, we have found that level of MDA is significantly less in the study group (p < 0.01).Thus, observed data would lead us...

Heart rnitochondrion ATPase activity, ADP:O ratio (P:O) and Respiratory Control (RCR) are compared between guinea pigs from high altitude (Morococha, 4540 m) (HA) and those from sea level (SL). The study comprised 60 male guinea pigs, 30 from each altitude level, with mean weight 400-450 grams. Heart mitochondria were obtained by differential centrifugation at 4°C. P:O ratio and RCR were measured polarographically by Tyler method, and ATP hydrolysis was done by Holton et al. Method. Mean values obtained for heart mitochondria at SL and HA, expressed in mmoles oxygen uptake/mg protein/hour, were, with Glutamate + Malate as subtrates, respectiveIy: P:O 2.90, RCR 6.02 and P:O 2.93, RCR 6.0; and, with Pyruvate + Malate as subtrates, respectively P:O 2.60, RCR 5.0 and P:O 2.70, RCR 4.80. ATPase activity was at SL and HA, respectively: 57.50 and 64.50 mmoles P /mg protein/hour. The results su...

Heart rnitochondrion ATPase activity, ADP:O ratio (P:O) and Respiratory Control (RCR) are compared between guinea pigs from high altitude (Morococha, 4540 m) (HA) and those from sea level (SL). The study comprised 60 male guinea pigs, 30 from each altitude level, with mean weight 400-450 grams. Heart mitochondria were obtained by differential centrifugation at 4°C. P:O ratio and RCR were measured polarographically by Tyler method, and ATP hydrolysis was done by Holton et al. Method. Mean values obtained for heart mitochondria at SL and HA, expressed in mmoles oxygen uptake/mg protein/hour, were, with Glutamate + Malate as subtrates, respectiveIy: P:O 2.90, RCR 6.02 and P:O 2.93, RCR 6.0; and, with Pyruvate + Malate as subtrates, respectively P:O 2.60, RCR 5.0 and P:O 2.70, RCR 4.80. ATPase activity was at SL and HA, respectively: 57.50 and 64.50 mmoles P /mg protein/hour. The results su...