Heart rnitochondrion ATPase activity, ADP:O ratio (P:O) and Respiratory Control (RCR) are compared between guinea pigs from high altitude (Morococha, 4540 m) (HA) and those from sea level (SL). The study comprised 60 male guinea pigs, 30 from each altitude level, with mean weight 400-450 grams. Heart mitochondria were obtained by differential centrifugation at 4°C. P:O ratio and RCR were measured polarographically by Tyler method, and ATP hydrolysis was done by Holton et al. Method. Mean values obtained for heart mitochondria at SL and HA, expressed in mmoles oxygen uptake/mg protein/hour, were, with Glutamate + Malate as subtrates, respectiveIy: P:O 2.90, RCR 6.02 and P:O 2.93, RCR 6.0; and, with Pyruvate + Malate as subtrates, respectively P:O 2.60, RCR 5.0 and P:O 2.70, RCR 4.80. ATPase activity was at SL and HA, respectively: 57.50 and 64.50 mmoles P /mg protein/hour. The results su...

Heart rnitochondrion ATPase activity, ADP:O ratio (P:O) and Respiratory Control (RCR) are compared between guinea pigs from high altitude (Morococha, 4540 m) (HA) and those from sea level (SL). The study comprised 60 male guinea pigs, 30 from each altitude level, with mean weight 400-450 grams. Heart mitochondria were obtained by differential centrifugation at 4°C. P:O ratio and RCR were measured polarographically by Tyler method, and ATP hydrolysis was done by Holton et al. Method. Mean values obtained for heart mitochondria at SL and HA, expressed in mmoles oxygen uptake/mg protein/hour, were, with Glutamate + Malate as subtrates, respectiveIy: P:O 2.90, RCR 6.02 and P:O 2.93, RCR 6.0; and, with Pyruvate + Malate as subtrates, respectively P:O 2.60, RCR 5.0 and P:O 2.70, RCR 4.80. ATPase activity was at SL and HA, respectively: 57.50 and 64.50 mmoles P /mg protein/hour. The results su...

The aim of this study was to determine semiquantitatively megakaryocyte glycogen and related parameters as an aid to diagnosis and treatment of idiopathic thrombocytopenic purpura (ITP). The tests were applied to 100 patients diagnosed with ITP from various hospitals and clinics, and 100 apparently healthy subjects who was gave informed consent according to the Declaration of Helsinki. Investigating the presence of glycogen in megakaryocytes of bone marrow smears using the method of peryodic acid-Schiff and complementary tests of hemostasis: platelets by the method of Rees-Ecker, mean platelet volume (MPV) using the automatic analyzer Sysmex XE-2100, prothrombin time, partial thromboplastin time (PTT), bleeding time, fibrinogen, hemoglobin and red blood cell count by the usual methods. We conclude that patients with ITP megakaryocyte glycogen had decreased (100%), alterations in some coa...

The aim of this study was to determine semiquantitatively megakaryocyte glycogen and related parameters as an aid to diagnosis and treatment of idiopathic thrombocytopenic purpura (ITP). The tests were applied to 100 patients diagnosed with ITP from various hospitals and clinics, and 100 apparently healthy subjects who was gave informed consent according to the Declaration of Helsinki. Investigating the presence of glycogen in megakaryocytes of bone marrow smears using the method of peryodic acid-Schiff and complementary tests of hemostasis: platelets by the method of Rees-Ecker, mean platelet volume (MPV) using the automatic analyzer Sysmex XE-2100, prothrombin time, partial thromboplastin time (PTT), bleeding time, fibrinogen, hemoglobin and red blood cell count by the usual methods. We conclude that patients with ITP megakaryocyte glycogen had decreased (100%), alterations in some coa...