In order to analyze the activity of the serum enzyme paraoxonase, phenotype distribution and its relationship with lipid profile and oxidative stress, in an elderly population from Cerro de Pasco city(4330 m), were studied blood serum of 30 men aged 60 years and 40 postmenopausal women. Paraoxon were used as substrate for measuring the activity of paraoxonase basal and stimulated with NaCl 1 M and measuring phenylacetate arylesterase activity. The lipid profile was measured using commercial kits, apolipoproteins A-1 and B by turbidimetric methods and malondialdehyde with thiobarbituric acid. The mean values of lipid profile, APO B, and malondialdehyde (expressed as TBARS) between men and women do not differ significantly. APO A-1 is significantly higher in women than in men. The activities of the PON 1 basal and stimulated not differ significantly between the two groups. There was signif...

In order to analyze the activity of the serum enzyme paraoxonase, phenotype distribution and its relationship with lipid profile and oxidative stress, in an elderly population from Cerro de Pasco city(4330 m), were studied blood serum of 30 men aged 60 years and 40 postmenopausal women. Paraoxon were used as substrate for measuring the activity of paraoxonase basal and stimulated with NaCl 1 M and measuring phenylacetate arylesterase activity. The lipid profile was measured using commercial kits, apolipoproteins A-1 and B by turbidimetric methods and malondialdehyde with thiobarbituric acid. The mean values of lipid profile, APO B, and malondialdehyde (expressed as TBARS) between men and women do not differ significantly. APO A-1 is significantly higher in women than in men. The activities of the PON 1 basal and stimulated not differ significantly between the two groups. There was signif...

Body weight, brain weight, and total fat, cholesterol, phospholipids and sphingolipids (sphingomyelines, cerebrosides, sulfatides, gangliosides) in brain were determined in 20 male guinea pigs, 10 native from sea-level zones (Lima, 150 m) and 10 from high altitude (Cerro de Pasco, 4380 m). The difference in body weight was significant, the high altitude guinea pig weight being reduced in 13.8% (p < 0.05). Neither brain weight nor total brain fat content showed significant differences. High altitude animals showed significant reductions: of cholesterol, 12.6% (p <0.01); of total phospholipids, 4,6 %; oj cerebrosides, 10.6% (p<0.001); and of sulfatides, 14.1% (p<0.001). The plasmalogens were increased in 22,6% (p<0.005). There were no significant differences in sphingomyelin and ganglioside levels. The differences found suggest that chronic exposure to high altitude hypoxia ...

Body weight, brain weight, and total fat, cholesterol, phospholipids and sphingolipids (sphingomyelines, cerebrosides, sulfatides, gangliosides) in brain were determined in 20 male guinea pigs, 10 native from sea-level zones (Lima, 150 m) and 10 from high altitude (Cerro de Pasco, 4380 m). The difference in body weight was significant, the high altitude guinea pig weight being reduced in 13.8% (p < 0.05). Neither brain weight nor total brain fat content showed significant differences. High altitude animals showed significant reductions: of cholesterol, 12.6% (p <0.01); of total phospholipids, 4,6 %; oj cerebrosides, 10.6% (p<0.001); and of sulfatides, 14.1% (p<0.001). The plasmalogens were increased in 22,6% (p<0.005). There were no significant differences in sphingomyelin and ganglioside levels. The differences found suggest that chronic exposure to high altitude hypoxia ...

Serum adenosine deaminase enzyme determinations were perfonned in 74 adult individuals of both sexes, consisting of 29 adult patients with active pulmonary tuberculosis, and 45 voluntary healthy individuals with no recent or past tuberculosis history, who constituted thecontrol group. The mean value of ADA enzyme serum activity in the group of patients with pulmonary tuberculosis was 47,89 + 6,05 U/L, and in the group of healthy individuals, 18.84 + 6.16 U/L. The difference between the means of serum ADA enzyme activity in the tuberculosis patients and the mean value of the control group was statistically significant (p < 0,001). The validation criteria showed the following results: sensitivity, 100%; specificity, 91.11%; efficiency, 94.59%; predictive value of positivity, 87,88%; predictive value of negativity, 100% .In the cases with high prevalence value, serum ADA test can be a si...

Serum adenosine deaminase enzyme determinations were perfonned in 74 adult individuals of both sexes, consisting of 29 adult patients with active pulmonary tuberculosis, and 45 voluntary healthy individuals with no recent or past tuberculosis history, who constituted thecontrol group. The mean value of ADA enzyme serum activity in the group of patients with pulmonary tuberculosis was 47,89 + 6,05 U/L, and in the group of healthy individuals, 18.84 + 6.16 U/L. The difference between the means of serum ADA enzyme activity in the tuberculosis patients and the mean value of the control group was statistically significant (p < 0,001). The validation criteria showed the following results: sensitivity, 100%; specificity, 91.11%; efficiency, 94.59%; predictive value of positivity, 87,88%; predictive value of negativity, 100% .In the cases with high prevalence value, serum ADA test can be a si...

Measurement of DNA, RNA and proteins in guinea pig testes, spleen, kidney, heart, liver and lungs, expressed per 100g et tissue, per organ, and as number of cells per organ was performed in 9 male, adult guinea pigs native from high altitude (Morococha, 4540m) and in 10 male, adult guinea pigs native from sea level (Lima, 150 m).Body weight was lower in high altitude guinea pigs, though not significantly; however the weight of most organs was higher in high altitude guinea pigs.High altitude animals showed higher kidney and lung protein contents per 100g wet tissue and per organ. Heart showed higher protein content per organ. Livershowed lower protein content per 100g tissue, but lower content per organ.DNA content was higher in high altitude guinea pig testes, kidneys, heart and lungs. RNA content was increased in most organs of high altitude guinea pigs, except the spleen.Cell number w...

Measurement of DNA, RNA and proteins in guinea pig testes, spleen, kidney, heart, liver and lungs, expressed per 100g et tissue, per organ, and as number of cells per organ was performed in 9 male, adult guinea pigs native from high altitude (Morococha, 4540m) and in 10 male, adult guinea pigs native from sea level (Lima, 150 m).Body weight was lower in high altitude guinea pigs, though not significantly; however the weight of most organs was higher in high altitude guinea pigs.High altitude animals showed higher kidney and lung protein contents per 100g wet tissue and per organ. Heart showed higher protein content per organ. Livershowed lower protein content per 100g tissue, but lower content per organ.DNA content was higher in high altitude guinea pig testes, kidneys, heart and lungs. RNA content was increased in most organs of high altitude guinea pigs, except the spleen.Cell number w...

We have determined antiaxidant enzyme activities of: Superoxide Dismutase (SOD), Glutatione Peroxidase (GPx) and Catalase (CAT); levels of malondialdehyde (MDA) and hydroperoxids as lipoperoxidation indicators, and phospholilpase activity as mediator of the lipoperoxidation in cerebral tissue of guinea pigs born and bred in Cerro de Pasco at 4 300 m (ALT), with respect to guinea pigs at sea level (NM), in order to detect real differences between both groups. With respect to activity of antioxidant enzymes, we have not found significant differences between both groups. In the study group (ALT) we have found significant differences in the activity of phospholipase and level of hydroperoxides (p < 0.01 Y P < 0.001. respectively) as compared to sea level group. Also, we have found that level of MDA is significantly less in the study group (p < 0.01).Thus, observed data would lead us...

In the present study, levels of malondialdehyde (MDA), end product of lipid peroxydation, and activity of catalase, a protective enzyme, in heart , kidney, liver and lung of guinea pigs born and bred in the altilude (Cullhuay, 3642 m), and another from sea level (La Molina, 238 m), were determined, in order to establish whether there exist any differences, and better understand of the oxydative stress in high altitude natives. Tissue levels of MDA were assessed by the n-methy-l-phenylindol method, and catalase enzyme activity was evaluated using the PURPALD colorimetric method. In tissue of heart, kidney, liver and lung of high altitude guinea pig were found MDA concentrations in mmoles/g protein: 0.309, 0.158, 0.133 and 0.257, values signifieantly higher in high altitude guinea pig than in those from sea level: 0.263, 0.115, 0.097 Y 0.201 (p < 0.05); and the value of catalase activit...

The Glucose-6-phosfate dehydrogenase (G6PD) specific activity was determined in adipose and hepatic tissues 0167 Holtzman, male, adults rats, born and bred at sea level. Of them, 48 were transported by automobile in three hours up to 4 540 m. above sea, and sacrificed in turn at 0,1,2,3,5,15 and 30 days after arrival. The other 19 were grouped in two classes: a control class of rats which never traveled, and another class of rats thal traveled around fer three hours, after which they were sacrificed.As a result, it was shown that the trip without ascenl did not cause any significant variation in adipose and hepatic tissue G6PD specific activity.In animals taken to the high altitude, it was observed a lower specific activity of adipose tissue G6PD than in those remaining at sea level. During the first two days the activity fell sharply, thereafter recovering slowly without actually return...

Placental contents of humidity, lipids, ashes, carbohydrates, DNA, RNA and proteins were observed in samples obtained immediately after normal delivery from two groups of pregnant women one in "Maternidad Municipal de Chorrillos" and other in a private Hospital in San Isidro.Selected subjects did not show any pathological condition: their ages varied from 20 to 35 years and had no more than two previous deliveries.It was found a significant difference between the means of lipid content, the first group having lower level that the second. The rest of components observed, including weight of placenta, newborn weigh, length and gestatinal age did not show any significant differences. lt can be assumed that 2(2) 103-110 (1999)both groups had similar nutritional levels during pregnancy.

We have determined antiaxidant enzyme activities of: Superoxide Dismutase (SOD), Glutatione Peroxidase (GPx) and Catalase (CAT); levels of malondialdehyde (MDA) and hydroperoxids as lipoperoxidation indicators, and phospholilpase activity as mediator of the lipoperoxidation in cerebral tissue of guinea pigs born and bred in Cerro de Pasco at 4 300 m (ALT), with respect to guinea pigs at sea level (NM), in order to detect real differences between both groups. With respect to activity of antioxidant enzymes, we have not found significant differences between both groups. In the study group (ALT) we have found significant differences in the activity of phospholipase and level of hydroperoxides (p < 0.01 Y P < 0.001. respectively) as compared to sea level group. Also, we have found that level of MDA is significantly less in the study group (p < 0.01).Thus, observed data would lead us...

In the present study, levels of malondialdehyde (MDA), end product of lipid peroxydation, and activity of catalase, a protective enzyme, in heart , kidney, liver and lung of guinea pigs born and bred in the altilude (Cullhuay, 3642 m), and another from sea level (La Molina, 238 m), were determined, in order to establish whether there exist any differences, and better understand of the oxydative stress in high altitude natives. Tissue levels of MDA were assessed by the n-methy-l-phenylindol method, and catalase enzyme activity was evaluated using the PURPALD colorimetric method. In tissue of heart, kidney, liver and lung of high altitude guinea pig were found MDA concentrations in mmoles/g protein: 0.309, 0.158, 0.133 and 0.257, values signifieantly higher in high altitude guinea pig than in those from sea level: 0.263, 0.115, 0.097 Y 0.201 (p < 0.05); and the value of catalase activit...

The Glucose-6-phosfate dehydrogenase (G6PD) specific activity was determined in adipose and hepatic tissues 0167 Holtzman, male, adults rats, born and bred at sea level. Of them, 48 were transported by automobile in three hours up to 4 540 m. above sea, and sacrificed in turn at 0,1,2,3,5,15 and 30 days after arrival. The other 19 were grouped in two classes: a control class of rats which never traveled, and another class of rats thal traveled around fer three hours, after which they were sacrificed.As a result, it was shown that the trip without ascenl did not cause any significant variation in adipose and hepatic tissue G6PD specific activity.In animals taken to the high altitude, it was observed a lower specific activity of adipose tissue G6PD than in those remaining at sea level. During the first two days the activity fell sharply, thereafter recovering slowly without actually return...

Placental contents of humidity, lipids, ashes, carbohydrates, DNA, RNA and proteins were observed in samples obtained immediately after normal delivery from two groups of pregnant women one in "Maternidad Municipal de Chorrillos" and other in a private Hospital in San Isidro.Selected subjects did not show any pathological condition: their ages varied from 20 to 35 years and had no more than two previous deliveries.It was found a significant difference between the means of lipid content, the first group having lower level that the second. The rest of components observed, including weight of placenta, newborn weigh, length and gestatinal age did not show any significant differences. lt can be assumed that 2(2) 103-110 (1999)both groups had similar nutritional levels during pregnancy.

The aim of this study was to assess the effect of high altitude exposure time on the oxidation of proteins and lipids from guinea pig brain tissue. Fifty guinea pigs (sea level natives) were taken up to Morococha (4540 m) and sacrificed on days 1, 3, 7 and 14, after arrival. Phospholipase A2 (PLA2) activity, levels of lipid hydroperoxides (LOOH) and malondialdehyde (TBARS) were determined as markers of lipid oxidation. Carbonyl groups (CG) were also determined as markers of protein oxidation. Activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were also determined. PLA2 activity increased until third day. LOOH, TBARS and CG levels were found to be increased the first day; LOOH levels showed a declining trend but TBARS levels remained high, CG concentration decreased below the control group levels on the third day. The activities o...

It was determined the levels of antioxidant enzymes: Glutathione Peroxidase (GPx), Superoxide Dismutase (SOD) and Catalase (CAT), and the concentratíon of Malondialdehyde (MDA) as an indicator of lipid peroxidatíon in erythrocytes of 60 apparently healthy people: 30 residents at high altitude (Cerro de Paseo, 4340 m) and 30 residents at sea level (Lima, 150 m). We found erythrocyte GPx levels higher in high altitude natives than in the sea level ones (p < .001). No statistically significant differencc was found between the val u es obtained for SOD in high altitude na ti ves and those of sea leveL The CAT activity was lower in subjccts of high altitude in relation to those of sea level (p < .001). High altitude native MDA levels also were lower than those obtained at sea level (p < .001). We concluded that Glutathione Peroxidase is the main antioxidant enzyme for the detoxific...

The aim of this study was to assess the effect of high altitude exposure time on the oxidation of proteins and lipids from guinea pig brain tissue. Fifty guinea pigs (sea level natives) were taken up to Morococha (4540 m) and sacrificed on days 1, 3, 7 and 14, after arrival. Phospholipase A2 (PLA2) activity, levels of lipid hydroperoxides (LOOH) and malondialdehyde (TBARS) were determined as markers of lipid oxidation. Carbonyl groups (CG) were also determined as markers of protein oxidation. Activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were also determined. PLA2 activity increased until third day. LOOH, TBARS and CG levels were found to be increased the first day; LOOH levels showed a declining trend but TBARS levels remained high, CG concentration decreased below the control group levels on the third day. The activities o...

Se determinó los niveles de las enzimas antioxidantes: glutation peroxidasa (GPx), su peróxido dismutasa (SOD) y catalasa(CAT); y la concentración de malondialdehído (MDA) como un indicador de la peroxidación lipídica, en eritrocitos de 60 personas aparentemente sanas: 30 residentes de la altura (Cerro de Paseo, 4340 m) y 30 residentes de nivel del mar (Lima,150m). Se encontró niveles eritrocitarios de GPx mayores en los nativos de altura que en los de nivel del mar (p < 0,001). No se encontró diferencia estadísticamente significativa entre los valores obtenidos para la SOD en los nativos de altura y en los de nivel del mar. La actividad de la CAT fue menor en los sujetos de altura en relación con los de nivel del mar(p < 0,001). Los niveles de MDA en los nativos de altura también fueron menores que los obtenidos a nivel del mar (p < 0,001). Se concluye que la glutati...