Objective: To determine the frequency and factors associated with Hepatitis B or C coinfection in HIV-infected population, in Callao Region, 2016. Methodology: Descriptive, cross-sectional, retrospective study that included samples of HIV infected persons previously confirmed by the HIV LRN, these samples came from the Callao Region, in 2016. A base was prepared and 87 samples were selected that met the inclusion criteria. These samples were performed with the ELISA test to determine the presence of the markers for HBsAg HBV and anti HCV-core HCV. For the data analysis, the statistical package SPSS version 23 was used. Since it is a descriptive prevalence study, inference or statistical estimation of the prevalence was applied. Descriptive statistics were performed; to establish frequencies and percentages, to correlate dependent and independent variables and correlation of variables thr...

Objectives: To standardize and validate a western blot test for the diagnosis of human immunodeficiency virus. Methods: A prospective observational study was carried out during 2017 and 2018. The western blot test was standardized, using the polyacrylamide gel electrophoresis technique with sodium dodecyl sulfate (SDS PAGE), being the nitrocellulose blot strips prepared with an Optimal HIV-1 antigen concentration of 2.71 µg / mm. The western blot was validated in the laboratory against 400 reference samples (300 sera and 100 plasmas): 200 positive and 200 negatives for antibodies against HIV-1, being the reference test the Immunoblot of the Fujirebio brand. Diagnostic performance parameters were estimated using Epidat v3.1 and Excel. Results: Eight important bands of the HIV-1 antigen were identified: p17, p24, p31, p39, gp41, p55, p66, and gp120. According to t...

El objetivo es determinar el nivel de correlación de la cuantificación de carga viral para VIH-1 mediante las técnicas Amplicor HIV-1 Monitor Versión Estándar y Sistema COBAS Ampliprep/COBAS Taqman 48 HIV-1; los materiales y métodos due que se realizó un estudio correlacional, se incluyó 152 muestras de plasma, de personas viviendo con VIH/SIDA que se encuentran en el Sistema de Monitoreo de TARGA del INS. Las muestras incluidas tenían carga viral en rango de 400 a 750,000 copias/mL, y se almacenaron a -20 ºC hasta 15 días antes de su procesamiento. Las muestras fueron procesadas por el método Amplicor HIV-1 que usa la técnica de RT-PCR, y con el método COBASAmpliprep/TaqMan HIV-1, tecnología automatizada que utiliza el PCR en tiempo real.

De acuerdo con las estadísticas del Ministerio de Salud, antes del inicio de este programa nacional, cerca de 7000 personas con VIH / SIDA necesitaban terapia antirretroviral en el Perú. Las prevalencias más elevadas se encuentran en Lima, Callao, Iquitos, Ica y el norte del país. Alrededor del 75% de los casos viven en Lima y Callao y el otro 25% en provincias.