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The Peruvian Andean Plateau, one of the main production areas of native varieties of Chenopodium quinoa, is exposed to abrupt decreases in environmental temperature, affecting crop production. Plant-growth-promoting rhizobacteria that tolerate low temperatures could be used as organic biofertilizers in this region. We aimed to bioprospect the native psychrotolerant bacteria of the quinoa rhizosphere in this region that show plant-growth-promoting traits. Fifty-one strains belonging to the quinoa rhizosphere were characterised; 73% of the total could grow at low temperatures (4, 6, and 15 °C), whose genetic diversity based on DNA amplification of interspersed repetitive elements (BOX) showed 12 different profiles. According to the 16S rRNA sequence, bacterial species belonging to the classes Beta-and Gammaproteobacteria were identified. Only three (6%) isolates identified as nonpathogeni...
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Treinta cepas de V. cholerae O1 Ogawa y 2 Inaba, aisladas durante el brote de 1998, fueron estudiados en relación a su sensibilidad frente a 10 antibióticos de uso clínico. Nueve cepas (28.1%) fueron resistentes de 1 hasta 6 antibióticos. El SIDS-PAGE de las cepas estudiadas exhibieron un patrón similar de bandas.
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Thirty strains of V. cholerae O1 Ogawa and 2 Inaba, isolated during the outbreak of 1998, were studied in relation to thier sensibility in front of 10 antibiotics of clinical use. Nine strains were determined (28.1%) resistant to 1 up to 6 antibiotics. The SIDS-PAGE of the studied strains exhibited similar pattern of bands.
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This work was partially funded by the AECID (Agencia Española de Cooperación Internacional para el Desarrollo) grant numbers A/3795/05, A/4892/06, A/9727/07 A/4817/06; ACCD (Agència Catalana de Cooperació al Desenvolupament, Generalitat de Catalunya) grant U2006; CCD-UPC (Centre de Cooperació per al Desenvolupament, Universitat Politècnica de Catalunya) to LJdV; and by the CONCYTEC (Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica, Perú) grant 189-2005-CONCYTEC-OAJ. JR's research is supported by grant CP05/0130 of the FIS (Fondo de Investigaciones Sanitarias). The authors thank M.J. Pons and Donna Pringle for assistance in the preparation of the final version of the manuscript. Conflict of interest: No conflict of interest to declare.