Detection of Bordetella pertussis using a PCR test in infants younger 3 than one year old hospitalized with whooping cough in five 4 Peruvian hospitals

• Autores: Castillo, María Esther, Bada, Carlos, Del Aguila ,Olguita, Petrozzi Helasvuo, Verónica, Casabona Ore, Verónica, Reyes, Isabel, Del Valle Mendoza, Juana Mercedes
• Formato: artículo
• Fecha de Publicación: 2015
• Institución: Universidad Peruana de Ciencias Aplicadas
• Repositorio: UPC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorioacademico.upc.edu.pe:10757/582607
• Enlace del recurso: https://doi.org/10.1016/j.ijid.2015.10.020; http://hdl.handle.net/10757/582607
• Nivel de acceso: acceso abierto
• Materia: Whooping cough; PCR; Peru; Bordetella pertussis

Objectives To report the incidence, epidemiology, and clinical features of Bordetella pertussis in Peruvian infants under 1 year old. Patients and methods A prospective cross-sectional study was conducted in five hospitals in Peru from January 2010 to July 2012. A total of 392 infants under 1 year old were admitted with a clinical diagnosis of whooping cough and tested for B. pertussis by PCR. Results The pertussis toxin and IS481 genes were detected in 39.54% (155/392) of the cases. Infants aged less than 3 months were the most affected, with a prevalence of 73.55% (114/155). The most common household contact was the mother, identified in 20% (31/155) of cases. Paroxysm of coughing (89.03%, 138/155), cyanosis (68.39%, 106/155), respiratory distress (67.09%, 104/155), and breastfeeding difficulties (39.35%, 61/155) were the most frequent symptoms reported. Conclusion An increase in pertussis cases has been reported in recent years in Peru, despite national immunization efforts. Surveillance with PCR for B. pertussis is essential, especially in infants less than 1 year old, in whom a higher rate of disease-related complications and higher mortality have been reported.