Caracterización sintomatológica y determinación de virus que infectan al cultivo de carica papaya l. en algunas zonas del norte del Perú

• Autor: Valderrama Alfaro, Shirley Medeleine
• Formato: tesis de maestría
• Fecha de Publicación: 2013
• Institución: Universidad Nacional de Trujillo
• Repositorio: UNITRU-Tesis
• Lenguaje: español
• OAI Identifier: oai:dspace.unitru.edu.pe:20.500.14414/4059
• Enlace del recurso: https://hdl.handle.net/20.500.14414/4059
• Nivel de acceso: acceso abierto
• Materia: Prsv, Indicadoras, Ncm-elisa, Carica papaya l, Rt-pcr, Norte del perú

The main objective of this assay was evaluated that virus is infecting papaya Carica papaya in northern Peru and have symptomatology characterization of them. The incidence and severity of virus was examined by diseases symptoms consist of mosaic, yellowing, chlorosis, leaf distortion (“frogs foot”), filiform leaf, blistering, greasy-appearing streak on stems and petioles, ringspot and streaking on fruits in farmers field of Paijan, Chepen, Motupe, Monsefú, Túcume, Tambogrande, Chulucanas and Corrales and samples with symptoms were collected. Our surveys conducted in northern Peru showed an incidence between 7 and 100 % and a severity between 8.3 and 77.5% according to evaluation scale 2 (new leaves with chlorosis and leaf distortion, mild mosaic, leaves with pallid reticulate, 3-5 leaf affected) and 3 (chlorosis, leaf apical distortion, severe mosaic, blistering and frogs foot). A range of indicators plants, six different plant species (Chenopodium murale, Ch. amaranticolor, Ch. quinoa, Cucumis melo “melon”, C. sativus “pepinillo” and C. pepo “zapallito italiano) along with the natural host, Papaya, were mechanically inoculated with isolates of different zones under study. Plants were evaluated per 45 days after inoculation. NCM-ELISA and RT-PCR tests to PRSV were done to selected papaya and selected indicator plants, which showed visual symptoms. Cucumis melo, C. sativus and Cucumis pepo showed systemic chlorosis. However, all the inoculated papaya plants exhibited mosaic, blistering, leaves with pallid reticulate, rugosity, leaf filiform and, reduction of foliar area. The results confirm the high and wide incidence of Papaya ringspot virus (PRSV) in some zones of northern Peru.