Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente.
Descripción del Articulo
Antimicrobial susceptibility was evaluated of Staphylococcus aureus from sausage hand-crafted, for which 20 crops were reactivated and prepared bacterial turbidity equivalent to a 0.5 tube of inoculants nephelometer Mac Farland evaluated; then seeded by surface with sterile swabs on Muller-Hinton ag...
Autor: | |
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Formato: | tesis de grado |
Fecha de Publicación: | 2016 |
Institución: | Universidad Nacional de Trujillo |
Repositorio: | UNITRU-Tesis |
Lenguaje: | español |
OAI Identifier: | oai:dspace.unitru.edu.pe:20.500.14414/9026 |
Enlace del recurso: | https://hdl.handle.net/20.500.14414/9026 |
Nivel de acceso: | acceso abierto |
Materia: | Susceptibilidad antimicrobiana, Staphylococcus aureus, chorizo. |
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dc.title.es_PE.fl_str_mv |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
title |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
spellingShingle |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. Orbegoso Noriega, Marietta Victoria Layli Susceptibilidad antimicrobiana, Staphylococcus aureus, chorizo. |
title_short |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
title_full |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
title_fullStr |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
title_full_unstemmed |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
title_sort |
Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente. |
author |
Orbegoso Noriega, Marietta Victoria Layli |
author_facet |
Orbegoso Noriega, Marietta Victoria Layli |
author_role |
author |
dc.contributor.advisor.fl_str_mv |
Llenque Díaz, Luis Alberto |
dc.contributor.author.fl_str_mv |
Orbegoso Noriega, Marietta Victoria Layli |
dc.subject.es_PE.fl_str_mv |
Susceptibilidad antimicrobiana, Staphylococcus aureus, chorizo. |
topic |
Susceptibilidad antimicrobiana, Staphylococcus aureus, chorizo. |
description |
Antimicrobial susceptibility was evaluated of Staphylococcus aureus from sausage hand-crafted, for which 20 crops were reactivated and prepared bacterial turbidity equivalent to a 0.5 tube of inoculants nephelometer Mac Farland evaluated; then seeded by surface with sterile swabs on Muller-Hinton agar and antibiotic discs were placed: vancomycin, rifampin, ciprofloxacin, doxycycline, ampicillin, clindamycin, erythromycin and gentamicin in each plate , incubated at 37 °C for 18 hours ; subsequently, the halos of growth inhibition were measured in millimeters and the degree of susceptibility (sensitive, resistant and medium) was determined with reference to the table qualification standards NCCLS, 2000. After processing the data obtained, it was observed that 100% of cultures were susceptible to vancomycin, gentamicin, erythromycin, rifampicin, ciprofloxacin and doxycycline. While 90% of cultures were sensitive to ampicillin, and 10% were resistant. Likewise, 55% of crops were sensitive to clindamycin, and 45% had intermediate susceptibility. Keywords: antimicrobial susceptibility, Staphylococcus aureus, sausage. |
publishDate |
2016 |
dc.date.accessioned.none.fl_str_mv |
10/25/2017 11:25 |
dc.date.available.none.fl_str_mv |
10/25/2017 11:25 |
dc.date.issued.fl_str_mv |
2016 |
dc.type.es_PE.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
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bachelorThesis |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.14414/9026 |
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https://hdl.handle.net/20.500.14414/9026 |
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SUNEDU |
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Tesis;T-3760 |
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info:eu-repo/semantics/openAccess |
dc.rights.uri.es_PE.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/pe/ |
eu_rights_str_mv |
openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/pe/ |
dc.publisher.es_PE.fl_str_mv |
UNIVERSIDAD NACIONAL DE TRUJILLO |
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Universidad Nacional de Trujillo Repositorio institucional - UNITRU |
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spelling |
Llenque Díaz, Luis AlbertoOrbegoso Noriega, Marietta Victoria Layli10/25/2017 11:2510/25/2017 11:252016https://hdl.handle.net/20.500.14414/9026Antimicrobial susceptibility was evaluated of Staphylococcus aureus from sausage hand-crafted, for which 20 crops were reactivated and prepared bacterial turbidity equivalent to a 0.5 tube of inoculants nephelometer Mac Farland evaluated; then seeded by surface with sterile swabs on Muller-Hinton agar and antibiotic discs were placed: vancomycin, rifampin, ciprofloxacin, doxycycline, ampicillin, clindamycin, erythromycin and gentamicin in each plate , incubated at 37 °C for 18 hours ; subsequently, the halos of growth inhibition were measured in millimeters and the degree of susceptibility (sensitive, resistant and medium) was determined with reference to the table qualification standards NCCLS, 2000. After processing the data obtained, it was observed that 100% of cultures were susceptible to vancomycin, gentamicin, erythromycin, rifampicin, ciprofloxacin and doxycycline. While 90% of cultures were sensitive to ampicillin, and 10% were resistant. Likewise, 55% of crops were sensitive to clindamycin, and 45% had intermediate susceptibility. Keywords: antimicrobial susceptibility, Staphylococcus aureus, sausage.Se evaluó la susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente, para lo cual se reactivaron los 20 cultivos y prepararon los inóculos bacterianos a una turbidez equivalente al tubo 0.5 del nefelómetro de Mac Farland; luego se sembró, por superficie, con hisopos estériles en agar Muller-Hinton, y se colocaron los discos de antibióticos: vancomicina, rifampicina, ciprofloxacino, doxiciclina, ampicilina, clindamicina, eritromicina y gentamicina en cada placa, se incubaron a 37° C por 18 horas; posteriormente, se midieron los halos de inhibición del crecimiento, en mm y se determinó el grado de susceptibilidad (Sensible, Resistente e Intermedia) teniendo como referencia la tabla de calificación de las normas del NCCLS, 2000. Después de procesar los datos obtenidos, se observó que el 100% de cultivos resultaron sensibles a vancomicina, gentamicina, eritromicina, rifampicina, ciprofloxacino y doxiciclina. Mientras que, el 90% de cultivos fueron sensibles a clindamicina, y el 45% tuvo susceptibilidad intermedia._x000D_ Palabras clave: susceptibilidad antimicrobiana, Staphylococcus aureus, chorizo.TesisspaUNIVERSIDAD NACIONAL DE TRUJILLOTesis;T-3760SUNEDUinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/pe/Universidad Nacional de TrujilloRepositorio institucional - UNITRUreponame:UNITRU-Tesisinstname:Universidad Nacional de Trujilloinstacron:UNITRUSusceptibilidad antimicrobiana, Staphylococcus aureus, chorizo.Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente.info:eu-repo/semantics/bachelorThesisTítulo ProfesionalBiólogo MicrobiólogoMicrobiologíaUniversidad Nacional de Trujillo.Facultad de Ciencias BiólogicasORIGINALOrbegoso Noriega, Marietta Victoria Layli.pdfOrbegoso Noriega, Marietta Victoria Layli.pdfapplication/pdf1763109https://dspace.unitru.edu.pe/bitstreams/bb637397-1cff-49c8-a7a8-056ee0e3fee0/download5517ee186989b6cffb22dd1f7dd166f6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://dspace.unitru.edu.pe/bitstreams/589581a1-3157-4d3a-89a7-49ba969a7db8/download8a4605be74aa9ea9d79846c1fba20a33MD5220.500.14414/9026oai:dspace.unitru.edu.pe:20.500.14414/90262024-04-21 13:22:46.952http://creativecommons.org/licenses/by-nc-nd/2.5/pe/info:eu-repo/semantics/openAccessopen.accesshttps://dspace.unitru.edu.peRepositorio Institucional - UNITRUrepositorios@unitru.edu.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 |
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