Actividad inhibitoria in vitro del extracto de Calliandra angustifolia Spruce ex Benth (bobinsana) y Piper aduncum L. (matico) sobre α - glucosidasa

• Autor: Castillo López, Pedro Luis
• Formato: tesis de grado
• Fecha de Publicación: 2022
• Institución: Universidad Nacional De La Amazonía Peruana
• Repositorio: UNAPIquitos-Institucional
• Lenguaje: español
• OAI Identifier: oai:repositorio.unapiquitos.edu.pe:20.500.12737/8693
• Enlace del recurso: https://hdl.handle.net/20.500.12737/8693
• Nivel de acceso: acceso abierto
• Materia: Inhibidores de enzimas; Alfa glucosidasa; Extractos vegetales; In vitro; Bobinsana; Calliandra angustifolia; Matico; Piper aduncum; https://purl.org/pe-repo/ocde/ford#3.01.05

Medicinal plants have therapeutic applications in the treatment of diabetes and currently an in vitro model has been developed to analyze plant species that exhibit hypoglycemic activity by inhibiting the enzyme ?-glucosidase. Objective: To determine the inhibitory activity in vitro of the extracts of Calliandra angustifolia Spruce ex Benth (bobinsana) and Piper aduncum L. (matico) on ? – glucosidase. Methodology: Samples were collected for convenience, micropulverized and subsequent preparation of extracts evaluated by spectroscopic analysis at concentrations of 100, 250, 500 and 1000 ug/mL where millipore water was used as a dilution medium and the drug acarbose as test control. Results: The ethanolic extract of bobinsana bark at a concentration of 100 ?g/mL showed better inhibition % than the ethanolic extracts of bobinsana and matico leaves; even against the positive control (acarbose). However, the bobinsana bark extract evaluated at concentrations of 500 and 1000 ?g/mL showed more than 50% inhibition of ?-glucosidase; being contrary to the extract of bobinsana leaves that only at the concentration of 1000 ?g/mL exceeded 50% inhibition; the ethanolic extract of matico leaves did not show an inhibition greater than 50% on ?-glucosidase. An IC50=424.71 ?g for the bobinsana bark extract indicated that at this concentration it produced inhibitory activity; against an IC50=968.43 ?g for extract of bobinsana leaves and an IC50=29778.28 ?g for extract of matico leaves. Conclusions: the extract of bobinsana bark showed a better IC50 than the extracts of bobinsana and matico leaves, since it was the most active inhibitor.