Tamizaje fitoquímico y actividad antibacteriana de Alchornea triplinervia (Sprengel) Muell Arg (zancudo caspi)

• Autores: Melena Vargas, Carmina, Moreno Fasanando, Saly Giovanna
• Formato: tesis de grado
• Fecha de Publicación: 2022
• Institución: Universidad Nacional De La Amazonía Peruana
• Repositorio: UNAPIquitos-Institucional
• Lenguaje: español
• OAI Identifier: oai:repositorio.unapiquitos.edu.pe:20.500.12737/8289
• Enlace del recurso: https://hdl.handle.net/20.500.12737/8289
• Nivel de acceso: acceso abierto
• Materia: Tamizaje; Antibacterianos; Extractos vegetales; Zancudo caspi; Alchornea triplinervia; https://purl.org/pe-repo/ocde/ford#3.01.05

Worldwide, bacterial resistance is a public health problem, being one of the main causes of therapeutic failure in infectious diseases. The objective of the present study was to determine the in vitro antibacterial activity of Alchornea triplinervia (Sprengel) Muell Arg "zancudo caspi" against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 and clinical isolates, and to identify the secondary metabolites in leaves and bark, through phytochemical screening. For phytochemical screening, the method of Chhabra, et al. Adapted and for antibacterial activity, the Disc Diffusion method (Kirby-Bauer) and CMI. The yield obtained was: leaf 11.88% and bark 3.22%. In the phytochemical screening, the presence of phenols, tannins, flavonoids, steroidal triterpenes and saponins was found; In the antibacterial activity according to the inhibition halo, the leaf extract presented an inhibition halo of 12 mm at 125 mg/mL and the bark 11 mm at 62.5 mg/mL, against S. aureus. Ampicillin, penicillin and oxacillin were used as positive control and ethanol as negative control. It is concluded that this plant species shows an abundant presence of flavonoids, phenols, tannins and a low amount of steroidal triterpenes and saponins. According to the inhibition halo obtained against S. aureus, this activity is considered to be intermediate and according to the minimum inhibitory concentration (MIC) it is inactive; likewise, the leaves and bark of A. triplinervia are inactive against P. aeruginosa and E. coli.