Cambios en la expresión de transportadores de glucosa de Taenia solium asociados al proceso de evaginación

• Autor: Castañeda Carpio, David
• Formato: tesis de grado
• Fecha de Publicación: 2021
• Institución: Universidad Peruana Cayetano Heredia
• Repositorio: UPCH-Institucional
• Lenguaje: español
• OAI Identifier: oai:repositorio.upch.edu.pe:20.500.12866/9060
• Enlace del recurso: https://hdl.handle.net/20.500.12866/9060
• Nivel de acceso: acceso abierto
• Materia: Metabolismo; Taenia solium; Evaginación; https://purl.org/pe-repo/ocde/ford#3.03.07; https://purl.org/pe-repo/ocde/ford#3.03.08

Taenia solium develops into a sexually mature flatworm exclusively in the human gut; its eggs are excreted with the host´s feces, contaminating water, soil, and food. The evagination of the scolex from the ingested larval cyst and the consequent cellular proliferation are critical processes for the parasite´s development and growth into the adult form. Changes in the metabolism and glucose transport in proliferating tissues and during regeneration have been reported for other flatworm species. In T. solium, glucose transporters TGTP1 and TGTP2 have been identified, as well as a hypothetical SGLT1-like sodium-glucose cotransporter in an aberrant, hyperproliferative larval form. However, it is not known if these transporters play a significant role in the parasite´s metamorphosis. We found variations at the mRNA level of glucose transporters using RT-qPCR on T. solium cysts obtained from pig muscle and incubated with taurocholic acid 0.1% (TA), a bile component that induces evagination. First, we identified the sequence for a T. solium SGLT1-like transporter homologous to transporters of the MFS superfamily from different species of flatworms. This transporter showed higher mRNA levels 24 h and 120 h after the incubation with TA, compared with cysts incubated without the inducer. The mRNA levels of TGTP1 also had a slight increase after incubation with TA. Furthermore, we identified a sequence for another SGLT1-like transporter in T. solium, homologous to transporters of the mammalian SLC5-6 superfamily. This work is a first step in understanding how glucose metabolism is involved in T. solium development. We propose to evaluate the expression of proteins related to the glycolytic pathway and the TCA cycle. Finally, the study of signaling pathways associated with metabolic regulation would provide more information about the nutritional requirements during parasite development.