Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024
Descripción del Articulo
The indiscriminate use of antimicrobials has led enterobacteria such as Escherichia coli to develop a variety of resistance mechanisms such as the production of extended-spectrum beta-lactamases (ESBL). Beta-lactam antimicrobials including penicillins, monobactams, and cephalosporins are among the m...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2024 |
| Institución: | Universidad Peruana Cayetano Heredia |
| Repositorio: | Revistas - Universidad Peruana Cayetano Heredia |
| Lenguaje: | español |
| OAI Identifier: | oai:revistas.upch.edu.pe:article/6867 |
| Enlace del recurso: | https://revistas.upch.edu.pe/index.php/STV/article/view/6867 |
| Nivel de acceso: | acceso abierto |
| id |
REVUPCH_2afe961421fa0145f0eaea144b3f37df |
|---|---|
| oai_identifier_str |
oai:revistas.upch.edu.pe:article/6867 |
| network_acronym_str |
REVUPCH |
| network_name_str |
Revistas - Universidad Peruana Cayetano Heredia |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 Presencia de Escherichia coli productora de betalactamasa de espectro extendido en el contenido fecal de ganado vacuno de dos mataderos de Lima, Perú, 2023-2024 Presença de Escherichia coli produtora de beta-lactamase de espectro estendido no conteúdo fecal de gado bovino de dois matadouros de Lima, Peru, 2023-2024 |
| title |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 |
| spellingShingle |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 Villafana, Lorena |
| title_short |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 |
| title_full |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 |
| title_fullStr |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 |
| title_full_unstemmed |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 |
| title_sort |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024 |
| dc.creator.none.fl_str_mv |
Villafana, Lorena Ramos-Delgado , Daphne D. Arévalo-Rodríguez, Karla Guevara Franco, Jose Alfredo Carhuallanqui , Andrea |
| author |
Villafana, Lorena |
| author_facet |
Villafana, Lorena Ramos-Delgado , Daphne D. Arévalo-Rodríguez, Karla Guevara Franco, Jose Alfredo Carhuallanqui , Andrea |
| author_role |
author |
| author2 |
Ramos-Delgado , Daphne D. Arévalo-Rodríguez, Karla Guevara Franco, Jose Alfredo Carhuallanqui , Andrea |
| author2_role |
author author author author |
| description |
The indiscriminate use of antimicrobials has led enterobacteria such as Escherichia coli to develop a variety of resistance mechanisms such as the production of extended-spectrum beta-lactamases (ESBL). Beta-lactam antimicrobials including penicillins, monobactams, and cephalosporins are among the most widely used in both animals and humans; Therefore, the presence of ESBL-producing E. coli (ESBL-EC) has the ability to hydrolyze these antimicrobials, leading to resistance against this class of agents, which represents a potencial threat to human and animal health. This study aimed to determine the presence of ESBL-EC in fecal samples from cattle at two slaughterhouses in Lima, Peru. A total of 260 fecal samples from cattle were collected at two slaughterhouses: S1 (n=134) and S2 (n=126). Antibiotic discs of cefotaxime (CTX) 30 µg, aztreonam (ATM) 30 µg, ceftazidime (CAZ) 30 µg, cefpodoxime (CPD) 10 µg and ceftriaxone (CRO) were used as screening method. While, antimicrobials cefotaxime - clavulanic acid (CTX-CLA) (30/10 µg) and ceftazidime - clavulanic acid (CAZ-CLA) (30/10 µg) were used in combined disc method for ESBL-EC phenotypic confirmation. The interpretation results follow the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Finally, was determined that 23.5% (61/260) of fecal sample presented ESBL-EC. Our results demonstrate that cattle intended for human consumption are carriers of ESBL-EC representing a potential public health risk. Keywords: ESBL-EC, antimicrobial resistance, cattle |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-12-10 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.upch.edu.pe/index.php/STV/article/view/6867 10.20453/stv.v13i2.6867 |
| url |
https://revistas.upch.edu.pe/index.php/STV/article/view/6867 |
| identifier_str_mv |
10.20453/stv.v13i2.6867 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.upch.edu.pe/index.php/STV/article/view/6867/6793 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Peruana Cayetano Heredia |
| publisher.none.fl_str_mv |
Universidad Peruana Cayetano Heredia |
| dc.source.none.fl_str_mv |
Salud y Tecnología Veterinaria; Vol. 13 No. 2 (2025): Julio-diciembre; e6867 Salud y Tecnología Veterinaria; Vol. 13 Núm. 2 (2025): Julio-diciembre; e6867 2312-3907 10.20453/stv.v13i2 reponame:Revistas - Universidad Peruana Cayetano Heredia instname:Universidad Peruana Cayetano Heredia instacron:UPCH |
| instname_str |
Universidad Peruana Cayetano Heredia |
| instacron_str |
UPCH |
| institution |
UPCH |
| reponame_str |
Revistas - Universidad Peruana Cayetano Heredia |
| collection |
Revistas - Universidad Peruana Cayetano Heredia |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1868349031706525696 |
| spelling |
Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024Presencia de Escherichia coli productora de betalactamasa de espectro extendido en el contenido fecal de ganado vacuno de dos mataderos de Lima, Perú, 2023-2024Presença de Escherichia coli produtora de beta-lactamase de espectro estendido no conteúdo fecal de gado bovino de dois matadouros de Lima, Peru, 2023-2024Villafana, LorenaRamos-Delgado , Daphne D.Arévalo-Rodríguez, KarlaGuevara Franco, Jose AlfredoCarhuallanqui , AndreaThe indiscriminate use of antimicrobials has led enterobacteria such as Escherichia coli to develop a variety of resistance mechanisms such as the production of extended-spectrum beta-lactamases (ESBL). Beta-lactam antimicrobials including penicillins, monobactams, and cephalosporins are among the most widely used in both animals and humans; Therefore, the presence of ESBL-producing E. coli (ESBL-EC) has the ability to hydrolyze these antimicrobials, leading to resistance against this class of agents, which represents a potencial threat to human and animal health. This study aimed to determine the presence of ESBL-EC in fecal samples from cattle at two slaughterhouses in Lima, Peru. A total of 260 fecal samples from cattle were collected at two slaughterhouses: S1 (n=134) and S2 (n=126). Antibiotic discs of cefotaxime (CTX) 30 µg, aztreonam (ATM) 30 µg, ceftazidime (CAZ) 30 µg, cefpodoxime (CPD) 10 µg and ceftriaxone (CRO) were used as screening method. While, antimicrobials cefotaxime - clavulanic acid (CTX-CLA) (30/10 µg) and ceftazidime - clavulanic acid (CAZ-CLA) (30/10 µg) were used in combined disc method for ESBL-EC phenotypic confirmation. The interpretation results follow the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Finally, was determined that 23.5% (61/260) of fecal sample presented ESBL-EC. Our results demonstrate that cattle intended for human consumption are carriers of ESBL-EC representing a potential public health risk. Keywords: ESBL-EC, antimicrobial resistance, cattleEl uso indiscriminado de antimicrobianos ha generado que enterobacterias como Escherichia coli desarrollen diversos mecanismos de resistencia como la producción de betalactamasas de espectro extendido (BLEE). Los antimicrobianos betalactámicos como las penicilinas, monobactámicos y cefalosporinas, se encuentran como los más utilizados tanto en animales como en humanos. Por lo tanto, la presencia de E. coli productoras de BLEE (EC-BLEE) con capacidad de hidrolizar a la mayoría de los betalactámicos representa una amenaza potencial para la salud humana y animal. El objetivo del estudio fue determinar la presencia de EC- BLEE en muestras de contenido fecal de ganado vacuno procedentes de dos mataderos de Lima, Perú. Se obtuvieron 260 muestras de contenido fecal de ganado vacuno de dos mataderos: M1 (n=134) y M2 (n=126). Los discos de antimicrobianos cefotaxima (CTX) 30 µg, aztreonam (ATM) 30 µg, ceftazidima (CAZ) 30 µg, cefpodoxima (CPD) 10 µg y ceftriaxona (CRO), se utilizaron como método de tamizaje. Mientras, que los discos de cefotaxima - ácido clavulánico (CTX-CLA) (30/10µg) y ceftazidima - ácido clavulánico (CAZ-CLA) (30/10 µg) se utilizaron para confirmar la presencia fenotípica de EC-BLEE. Los resultados de la interpretación siguieron las directrices del Instituto de Estándares Clínicos y de Laboratorio (CLSI). Finalmente, se determinó que el 23.5% (61/260) de las muestras fecales presentaban EC-BLEE. Nuestros resultados demuestran que el ganado vacuno destinado al consumo humano es portador de EC-BLEE, lo que representa un riesgo potencial para la salud pública. Palabras Clave: EC- BLEE, resistencia antimicrobiana, ganado vacuno.O uso indiscriminado de antimicrobianos levou enterobactérias como Escherichia coli a desenvolver vários mecanismos de resistência, como a produção de beta-lactamases de espectro estendido (ESBLs). Antimicrobianos beta-lactâmicos, como penicilinas, monobactâmicos e cefalosporinas, são os mais comumente usados em animais e humanos. Portanto, a presença de E. coli produtora de ESBL (ESBL-EC), capaz de hidrolisar a maioria dos beta-lactâmicos, representa uma ameaça potencial à saúde humana e animal. O objetivo deste estudo foi determinar a presença de ESBL-EC em amostras fecais de bovinos de dois matadouros em Lima, Peru. Um total de 260 amostras fecais de bovinos foram obtidas de dois matadouros: M1 (n = 134) e M2 (n = 126). Os discos antimicrobianos cefotaxima (CTX) 30 µg, aztreonam (ATM) 30 µg, ceftazidima (CAZ) 30 µg, cefpodoxima (CPD) 10 µg e ceftriaxona (CRO) foram utilizados como métodos de triagem. Enquanto isso, discos de cefotaxima-ácido clavulânico (CTX-CLA) (30/10 µg) e ceftazidima-ácido clavulânico (CAZ-CLA) (30/10 µg) foram utilizados para confirmar a presença fenotípica de ESBL-EC. A interpretação dos resultados seguiu as diretrizes do Clinical and Laboratory Standards Institute (CLSI). Ao final, 23,5% (61/260) das amostras fecais apresentaram ESBL-EC. Nossos resultados demonstram que bovinos destinados ao consumo humano são portadores de ESBL-EC, o que representa um risco potencial à saúde pública. Palavras-chave: ESBL-EC, resistência antimicrobiana, bovinos.Universidad Peruana Cayetano Heredia2024-12-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.upch.edu.pe/index.php/STV/article/view/686710.20453/stv.v13i2.6867Salud y Tecnología Veterinaria; Vol. 13 No. 2 (2025): Julio-diciembre; e6867Salud y Tecnología Veterinaria; Vol. 13 Núm. 2 (2025): Julio-diciembre; e68672312-390710.20453/stv.v13i2reponame:Revistas - Universidad Peruana Cayetano Herediainstname:Universidad Peruana Cayetano Herediainstacron:UPCHspahttps://revistas.upch.edu.pe/index.php/STV/article/view/6867/6793Derechos de autor 2025 Lorena Villafana, Ramos-Delgado Daphne D., Andrea Carhuallanqui , Karla Arévalo -Rodríguez, Jose Alfredo Guevara Francohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:revistas.upch.edu.pe:article/68672025-12-12T23:26:21Z |
| score |
12.623381 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
