The indiscriminate use of antimicrobials has led enterobacteria such as Escherichia coli to develop a variety of resistance mechanisms such as the production of extended-spectrum beta-lactamases (ESBL). Beta-lactam antimicrobials including penicillins, monobactams, and cephalosporins are among the most widely used in both animals and humans; Therefore, the presence of ESBL-producing E. coli (ESBL-EC) has the ability to hydrolyze these antimicrobials, leading to resistance against this class of agents, which represents a potencial threat to human and animal health. This study aimed to determine the presence of ESBL-EC in fecal samples from cattle at two slaughterhouses in Lima, Peru. A total of 260 fecal samples from cattle were collected at two slaughterhouses: S1 (n=134) and S2 (n=126). Antibiotic discs of cefotaxime (CTX) 30 µg, aztreonam (ATM) 30 µg, ceftazidime (CAZ) 30 µg, cefpod...