Presence of extended-spectrum beta-lactamase–producing Escherichia coli in fecal content of cattle from two slaughterhouses in Lima, Peru, 2023–2024

• Autores: Villafana, Lorena, Ramos-Delgado , Daphne D., Arévalo-Rodríguez, Karla, Guevara Franco, Jose Alfredo, Carhuallanqui , Andrea
• Formato: artículo
• Fecha de Publicación: 2024
• Institución: Universidad Peruana Cayetano Heredia
• Repositorio: Revistas - Universidad Peruana Cayetano Heredia
• Lenguaje: español
• OAI Identifier: oai:revistas.upch.edu.pe:article/6867
• Enlace del recurso: https://revistas.upch.edu.pe/index.php/STV/article/view/6867
• Nivel de acceso: acceso abierto

The indiscriminate use of antimicrobials has led enterobacteria such as Escherichia coli to develop a variety of resistance mechanisms such as the production of extended-spectrum beta-lactamases (ESBL). Beta-lactam antimicrobials including penicillins, monobactams, and cephalosporins are among the most widely used in both animals and humans; Therefore, the presence of ESBL-producing E. coli (ESBL-EC) has the ability to hydrolyze these antimicrobials, leading to resistance against this class of agents, which represents a potencial threat to human and animal health. This study aimed to determine the presence of ESBL-EC in fecal samples from cattle at two slaughterhouses in Lima, Peru. A total of 260 fecal samples from cattle were collected at two slaughterhouses: S1 (n=134) and S2 (n=126). Antibiotic discs of cefotaxime (CTX) 30 µg, aztreonam (ATM) 30 µg, ceftazidime (CAZ) 30 µg, cefpodoxime (CPD) 10 µg and ceftriaxone (CRO) were used as screening method. While, antimicrobials cefotaxime - clavulanic acid (CTX-CLA) (30/10 µg) and ceftazidime - clavulanic acid (CAZ-CLA) (30/10 µg) were used in combined disc method for ESBL-EC phenotypic confirmation. The interpretation results follow the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Finally, was determined that 23.5% (61/260) of fecal sample presented ESBL-EC. Our results demonstrate that cattle intended for human consumption are carriers of ESBL-EC representing a potential public health risk.   Keywords: ESBL-EC, antimicrobial resistance, cattle