Molecular sexing from stools in spectacled bear (Tremarctos ornatus)

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The spectacled bear (Tremarctos ornatus) is a vulnerable species because of hunting and destruction of its environment. Also, it is difficult to monitor in the wild hampering the management and research efforts and therefore, the use of non-invasive samples such as faeces becomes a useful tool. The...

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Detalles Bibliográficos
Autores: Caselli S., Caselli S., Maturrano H., Lenin
Formato: artículo
Fecha de Publicación:2016
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/11641
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641
Nivel de acceso:acceso abierto
Materia:spectacled bear
sexing
amelogenin gene
SRY
PCR
faeces
oso de anteojos
sexado
gen de amelogenina
heces
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spelling Molecular sexing from stools in spectacled bear (Tremarctos ornatus)Sexaje Molecular a Partir de Heces en Osos de Anteojos (Tremarctos ornatus)Caselli S., Caselli S.Maturrano H., Leninspectacled bearsexingamelogenin geneSRYPCRfaecesoso de anteojossexadogen de amelogeninaSRYPCRhecesThe spectacled bear (Tremarctos ornatus) is a vulnerable species because of hunting and destruction of its environment. Also, it is difficult to monitor in the wild hampering the management and research efforts and therefore, the use of non-invasive samples such as faeces becomes a useful tool. The aim of this study was to develop a technique for sexing by the Polymerase Chain Reaction (PCR) using DNA extracted from exfoliated colon cells present in faecal samples. Seventeen samples (from 10 females and 7 males) were collected from bears of known sex reared in captivity in four institutions in Lima Peru. DNA extraction was done by using a specific kit for faecal samples. The concentration of extracted DNA varied between 20 to 30 ng. Using PCR, two gene sequences were evaluated: amelogenin with primers SE47-SE48 and sex-determining region in the Y chromosome (SRY) with primers SRYB5-SRYB3. Sex determination by the amelogenin gene was achieved in all bears (100%), whereas failed in all samples when using the SRY gene primers. It is concluded that there is 100% match with the amelogenin gene between the known and detected sex using the PCR of DNA extracted from faeces.El oso de anteojos (Tremarctos ornatus) se encuentra vulnerable debido a la caza y destrucción de su medio ambiente y es de difícil observación en vida libre, lo que obstaculiza su manejo e investigación, por lo que el uso de muestras no invasivas como heces se convierte en una gran herramienta. El objetivo del presente estudio fue adaptar una técnica de sexado mediante la reacción en cadena de la polimerasa (PCR) de ADN extraído de células exfoliadas del colon presentes en muestras de heces. Se utilizaron 17 muestras (10 de hembras y 7 de machos) de osos de sexo conocido y criados en cautiverio en cuatro instituciones de la zona de Lima, Perú. Para la extracción de ADN se utilizó un kit comercial específico para heces. Se logró extraer de 20 a 30 ng de ADN de cada muestra. Mediante PCR se evaluaron dos secuencias de genes: amelogenina con los cebadores SE47-SE48 y la región determinante del sexo en el cromosoma Y (SRY) con los cebadores SRYB3- SRYB5. Con el gen de amelogenina se logró sexar a los 17 osos (100%), mientras que con el gen SRY no se logró el sexado. Se concluye que con el gen amelogenina hay una coincidencia de 100% entre el sexo conocido y el sexo hallado mediante PCR de ADN extraído de heces.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2016-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1164110.15381/rivep.v27i2.11641Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 252-258Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 252-2581682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641/10644Derechos de autor 2016 Caselli S. Caselli S., Lenin Maturrano H.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/116412017-02-20T16:35:40Z
dc.title.none.fl_str_mv Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
Sexaje Molecular a Partir de Heces en Osos de Anteojos (Tremarctos ornatus)
title Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
spellingShingle Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
Caselli S., Caselli S.
spectacled bear
sexing
amelogenin gene
SRY
PCR
faeces
oso de anteojos
sexado
gen de amelogenina
SRY
PCR
heces
title_short Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_full Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_fullStr Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_full_unstemmed Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_sort Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
dc.creator.none.fl_str_mv Caselli S., Caselli S.
Maturrano H., Lenin
author Caselli S., Caselli S.
author_facet Caselli S., Caselli S.
Maturrano H., Lenin
author_role author
author2 Maturrano H., Lenin
author2_role author
dc.subject.none.fl_str_mv spectacled bear
sexing
amelogenin gene
SRY
PCR
faeces
oso de anteojos
sexado
gen de amelogenina
SRY
PCR
heces
topic spectacled bear
sexing
amelogenin gene
SRY
PCR
faeces
oso de anteojos
sexado
gen de amelogenina
SRY
PCR
heces
description The spectacled bear (Tremarctos ornatus) is a vulnerable species because of hunting and destruction of its environment. Also, it is difficult to monitor in the wild hampering the management and research efforts and therefore, the use of non-invasive samples such as faeces becomes a useful tool. The aim of this study was to develop a technique for sexing by the Polymerase Chain Reaction (PCR) using DNA extracted from exfoliated colon cells present in faecal samples. Seventeen samples (from 10 females and 7 males) were collected from bears of known sex reared in captivity in four institutions in Lima Peru. DNA extraction was done by using a specific kit for faecal samples. The concentration of extracted DNA varied between 20 to 30 ng. Using PCR, two gene sequences were evaluated: amelogenin with primers SE47-SE48 and sex-determining region in the Y chromosome (SRY) with primers SRYB5-SRYB3. Sex determination by the amelogenin gene was achieved in all bears (100%), whereas failed in all samples when using the SRY gene primers. It is concluded that there is 100% match with the amelogenin gene between the known and detected sex using the PCR of DNA extracted from faeces.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641
10.15381/rivep.v27i2.11641
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641
identifier_str_mv 10.15381/rivep.v27i2.11641
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641/10644
dc.rights.none.fl_str_mv Derechos de autor 2016 Caselli S. Caselli S., Lenin Maturrano H.
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2016 Caselli S. Caselli S., Lenin Maturrano H.
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 252-258
Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 252-258
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.932913
Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
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