Inmunohistochemical Identification of Langerhans in Human Cornea

• Autores: Sedano Gelvet, Eduardo, Victorio, John, Neira, Carlos, Rojas, César
• Formato: artículo
• Fecha de Publicación: 1997
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/4674
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4674
• Nivel de acceso: acceso abierto
• Materia: Langerhans cells; Human cornea; Immunohistochemistry; Streptavidin-biotin peroxidase; Protein S-100; Muramidase; Células de Langerhans; Córnea humana; Inmunohistoquímica; Streptavidina-biotina peroxidasa; proteína S-100; muramidasa

We have achieved the identification of Langerhans cells in human cornea epithelium cross sections, fixed in Zenker’s liquid and embedded in paraffin, according to streptavidin-biotin peroxidase immunohistochemical method, in order to demonstrate protein S-100 and muramidase (lisozime) presence in them.Langerhans cells were identified easily from the rest of epithelial cells because they were the only elements protein S-100+ and muramidase+, which were stained light brown and showed rounded outlines with one or two prolongations.Langerhans cells were numerous in the limbic zone and null in the central region.