Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)

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The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of...

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Detalles Bibliográficos
Autores: Contreras, Winnie, Ruiz García, Luis, Santiani, Alexei
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/17548
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548
Nivel de acceso:acceso abierto
Materia:Vicugna pacos
cryopreservation
spermatozoa
epididymis
dimethylacetamide
dimethylformamide
cytometry
criopreservación
espermatozoides
epidídimo
dimetilacetamida
dimetilformamida
citometría
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spelling Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)Evaluación de dimetilacetamida y dimetilformamida como agentes crioprotectores para espermatozoides epididimarios de alpaca (Vicugna pacos)Contreras, WinnieRuiz García, LuisSantiani, AlexeiVicugna pacoscryopreservationspermatozoaepididymisdimethylacetamidedimethylformamidecytometryVicugna pacoscriopreservaciónespermatozoidesepidídimodimetilacetamidadimetilformamidacitometríaThe aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. 111/5000. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found.El objetivo del presente estudio fue determinar el efecto de dos tipos (dimetilacetamida [DMA] y dimetilformamida [DMF]) y tres concentraciones (1, 3.5, 7%) de agentes crioprotectores sobre parámetros espermáticos durante el proceso de criopreservación de espermatozoides epididimarios de alpaca. Se empleó un diseño factorial 2 x 3 formado por los dos crioprotectores y las tres concentraciones. Se evaluaron los parámetros de viabilidad espermática (SYBR-14/PI) y potencial de membrana mitocondrial (MitoTracker Deep Red FM) mediante citometría de flujo y motilidad pos-descongelación por microscopía de muestras de material espermático de 40 epidídimos. Se encontró un mayor porcentaje de motilidad pos-descongelamiento con DMA (14%) que con DMF (10%) (p=0.001). Además, hubo efecto significativo de la concentración del crioprotector en todos los parámetros evaluados (p<0.001), siendo mejor en las concentraciones de 1 y 3.5% que con 7%. No se halló efecto significativo de la interacción crioprotector-concentración.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1754810.15381/rivep.v31i1.17548Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 1 (2020); e17548Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 1 (2020); e175481682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548/14813Derechos de autor 2020 Winnie Contreras, Luis Ruiz García, Alexei Santianihttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/175482020-03-30T00:45:29Z
dc.title.none.fl_str_mv Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
Evaluación de dimetilacetamida y dimetilformamida como agentes crioprotectores para espermatozoides epididimarios de alpaca (Vicugna pacos)
title Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
spellingShingle Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
Contreras, Winnie
Vicugna pacos
cryopreservation
spermatozoa
epididymis
dimethylacetamide
dimethylformamide
cytometry
Vicugna pacos
criopreservación
espermatozoides
epidídimo
dimetilacetamida
dimetilformamida
citometría
title_short Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
title_full Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
title_fullStr Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
title_full_unstemmed Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
title_sort Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
dc.creator.none.fl_str_mv Contreras, Winnie
Ruiz García, Luis
Santiani, Alexei
author Contreras, Winnie
author_facet Contreras, Winnie
Ruiz García, Luis
Santiani, Alexei
author_role author
author2 Ruiz García, Luis
Santiani, Alexei
author2_role author
author
dc.subject.none.fl_str_mv Vicugna pacos
cryopreservation
spermatozoa
epididymis
dimethylacetamide
dimethylformamide
cytometry
Vicugna pacos
criopreservación
espermatozoides
epidídimo
dimetilacetamida
dimetilformamida
citometría
topic Vicugna pacos
cryopreservation
spermatozoa
epididymis
dimethylacetamide
dimethylformamide
cytometry
Vicugna pacos
criopreservación
espermatozoides
epidídimo
dimetilacetamida
dimetilformamida
citometría
description The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. 111/5000. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found.
publishDate 2020
dc.date.none.fl_str_mv 2020-03-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548
10.15381/rivep.v31i1.17548
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548
identifier_str_mv 10.15381/rivep.v31i1.17548
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548/14813
dc.rights.none.fl_str_mv Derechos de autor 2020 Winnie Contreras, Luis Ruiz García, Alexei Santiani
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Winnie Contreras, Luis Ruiz García, Alexei Santiani
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 1 (2020); e17548
Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 1 (2020); e17548
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238233166053376
score 13.958958
Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
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