Preimplantational embryo development in in vitro fertilization cycles with oocyte donation and genetic studies

• Autores: Villanueva, Pamela, Portella, Jimmy, López, Rosmary, Chávez, Nathaly, Villalobos, Amalia, Noriega-Hoces, Luis, Guzmán, Luis
• Formato: artículo
• Fecha de Publicación: 2017
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/13762
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/13762
• Nivel de acceso: acceso abierto
• Materia: Embryo development; Genetics; In vitro Fertilization; Preimplantational Genetic Study.; Desarrollo embrionario; Genética; Fertilización In vitro; Estudio Genético Preimplantacional.

Introduction: Preimplantational genetic studies are used to achieve a better implantation rate and live birth, as well as to decrease the abortion rate; these techniques should be evaluated. Objective: To evaluate preimplantational embryo development in donor oocyte in vitro fertilization cycles with genetic studies. Design: Retrospective cohort study. Setting: Pranor Laboratory, 2008-2013. Material: In vitro fertilization with ovodonation (IVF-OD) cycles. Interventions.: 2 077 cycles of IVF-OD were evaluated, separated in 3 cohorts: Cohort 1, cycles with embryo biopsy on day 3, by means of an incision in the zona pellucida (ZP), for excision of a blastomer (n = 527); Cohort 2, cycles with a laser incision at day 4 of embryo development, for biopsy of the trophoblast in the blastocyst stage (n = 131) and; cohort 3, FIV-OD cycles without any intervention (n = 1 419). Main outcome measures: Fertilization rate, blastulation rate. Results: There was no difference in the fertilization rate of the three groups studied (75.0%, 74.6% and 75.9% respectively, p = 0.31). Blastulation rate in cohort 1, was 42.5%, whereas cohort 3 was 47% (RR = 1.085, CI = 1.051-1.120; p <0.0001). In addition, cohort 2 had 51.9%, with statistical significance, which prevents from non-blastulation risk compared with cohort 3 (RR = 0.906; IC = 0.851-0.965; p = 0.0017). Conclusions:  Preimplantational embryo development would improve blastocyst formation when laser is performed on day 4. Further studies are needed to confirm our results.