Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia

Descripción del Articulo

The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle faeces from the municipality of Sincelejo, Sucre, Colombia. A direct bacteriological study was performed by microscopy techniques and polymerase chain reaction (PCR) for pools of f...

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Detalles Bibliográficos
Autores: Caraballo Blanco, Libardo, Castellar Martínez, Anais, Pardo Pérez, Enrique
Formato: artículo
Fecha de Publicación:2018
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/14111
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111
Nivel de acceso:acceso abierto
Materia:Mycobacterium avium subsp paratuberculosis
PCR
cattle diseases
Sincelejo
paratuberculosis
Mycobacterium avium subsp. paratuberculosis
enfermedades del ganado
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spelling Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, ColombiaMycobacterium avium subespecie paratuberculosis en heces de bovinos del municipio de Sincelejo, Sucre, ColombiaCaraballo Blanco, LibardoCastellar Martínez, AnaisPardo Pérez, EnriqueMycobacterium avium subsp paratuberculosisPCRcattle diseasesSincelejoparatuberculosisMycobacterium avium subsp. paratuberculosisPCRenfermedades del ganadoSincelejoparatuberculosisThe objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle faeces from the municipality of Sincelejo, Sucre, Colombia. A direct bacteriological study was performed by microscopy techniques and polymerase chain reaction (PCR) for pools of faeces with two molecular markers, one specific for MAP (IS900) and another based on 16S of the genus Mycobacterium. The presence of acid-fast bacilli occurred in 21.1% of the faecal samples stained with Ziehl Neelsen. Three pools were positive with IS900 and three with 16S. The sequencing indicated the presence of three species of bacteria: MAP, Mycobacterium abscessus and Dietzia timorensis. The presence of MAP in the department of Sucre is identified for the first time.El objetivo del estudio fue determinar la presencia de Mycobacterium avium subspecie paratuberculosis (MAP) en heces de bovinos del municipio de Sincelejo, Sucre, Colombia. Se realizó un estudio bacteriológico directo por técnicas de microscopía y reacción en cadena de la polimerasa (PCR) para pooles de heces con dos marcadores moleculares, uno específico para MAP (IS900) y otro basado en 16S del género Mycobacterium. La presencia de bacilos ácido-alcohol resistentes (BAAR) se dio en el 21.1% de las muestras de heces teñidas con Ziehl Neelsen. Tres pooles resultaron positivos con IS900 y tres con 16S. La secuenciación indicó la presencia de tres especies de bacterias: MAP, Mycobacterium abscessus y Dietzia timorensis. Se identifica por primera vez la presencia de MAP en el departamento de Sucre.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-09-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfimage/x-ms-bmpimage/x-ms-bmpimage/x-ms-bmpimage/x-ms-bmpimage/x-ms-bmptext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1411110.15381/rivep.v29i3.14111Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 3 (2018); 987-995Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 3 (2018); 987-9951682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13097https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13602https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13603https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13604https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13605https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13606https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13908Derechos de autor 2018 Libardo Enrique Caraballohttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/141112018-12-21T09:58:01Z
dc.title.none.fl_str_mv Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
Mycobacterium avium subespecie paratuberculosis en heces de bovinos del municipio de Sincelejo, Sucre, Colombia
title Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
spellingShingle Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
Caraballo Blanco, Libardo
Mycobacterium avium subsp paratuberculosis
PCR
cattle diseases
Sincelejo
paratuberculosis
Mycobacterium avium subsp. paratuberculosis
PCR
enfermedades del ganado
Sincelejo
paratuberculosis
title_short Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
title_full Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
title_fullStr Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
title_full_unstemmed Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
title_sort Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
dc.creator.none.fl_str_mv Caraballo Blanco, Libardo
Castellar Martínez, Anais
Pardo Pérez, Enrique
author Caraballo Blanco, Libardo
author_facet Caraballo Blanco, Libardo
Castellar Martínez, Anais
Pardo Pérez, Enrique
author_role author
author2 Castellar Martínez, Anais
Pardo Pérez, Enrique
author2_role author
author
dc.subject.none.fl_str_mv Mycobacterium avium subsp paratuberculosis
PCR
cattle diseases
Sincelejo
paratuberculosis
Mycobacterium avium subsp. paratuberculosis
PCR
enfermedades del ganado
Sincelejo
paratuberculosis
topic Mycobacterium avium subsp paratuberculosis
PCR
cattle diseases
Sincelejo
paratuberculosis
Mycobacterium avium subsp. paratuberculosis
PCR
enfermedades del ganado
Sincelejo
paratuberculosis
description The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle faeces from the municipality of Sincelejo, Sucre, Colombia. A direct bacteriological study was performed by microscopy techniques and polymerase chain reaction (PCR) for pools of faeces with two molecular markers, one specific for MAP (IS900) and another based on 16S of the genus Mycobacterium. The presence of acid-fast bacilli occurred in 21.1% of the faecal samples stained with Ziehl Neelsen. Three pools were positive with IS900 and three with 16S. The sequencing indicated the presence of three species of bacteria: MAP, Mycobacterium abscessus and Dietzia timorensis. The presence of MAP in the department of Sucre is identified for the first time.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111
10.15381/rivep.v29i3.14111
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111
identifier_str_mv 10.15381/rivep.v29i3.14111
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13097
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13602
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13603
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13604
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13605
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13606
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14111/13908
dc.rights.none.fl_str_mv Derechos de autor 2018 Libardo Enrique Caraballo
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2018 Libardo Enrique Caraballo
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
image/x-ms-bmp
image/x-ms-bmp
image/x-ms-bmp
image/x-ms-bmp
image/x-ms-bmp
text/html
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 3 (2018); 987-995
Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 3 (2018); 987-995
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.95948
Mycobacterium avium subsp. paratuberculosis in bovine faeces from the municipality of Sincelejo, Sucre, Colombia
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