The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle faeces from the municipality of Sincelejo, Sucre, Colombia. A direct bacteriological study was performed by microscopy techniques and polymerase chain reaction (PCR) for pools of faeces with two molecular markers, one specific for MAP (IS900) and another based on 16S of the genus Mycobacterium. The presence of acid-fast bacilli occurred in 21.1% of the faecal samples stained with Ziehl Neelsen. Three pools were positive with IS900 and three with 16S. The sequencing indicated the presence of three species of bacteria: MAP, Mycobacterium abscessus and Dietzia timorensis. The presence of MAP in the department of Sucre is identified for the first time.

The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle faeces from the municipality of Sincelejo, Sucre, Colombia. A direct bacteriological study was performed by microscopy techniques and polymerase chain reaction (PCR) for pools of faeces with two molecular markers, one specific for MAP (IS900) and another based on 16S of the genus Mycobacterium. The presence of acid-fast bacilli occurred in 21.1% of the faecal samples stained with Ziehl Neelsen. Three pools were positive with IS900 and three with 16S. The sequencing indicated the presence of three species of bacteria: MAP, Mycobacterium abscessus and Dietzia timorensis. The presence of MAP in the department of Sucre is identified for the first time.

The objective of this study was to investigate the genetic variability of criollo horses (Equus caballus) by genes associated with the coat. Fields visits were done between January and May 2016 in five populations of the municipality of Valencia, Colombia. The phenotypic characterization of the adult animals present in the farms was performed in relation to the autosomal markers of morphological codification Extension, Agouti, Cream, Gris, White, Tobiano, Overo and Roan. The results showed absence of the markers White and Overo, whereas the markers Extension and Agouti had the largest frequencies, possibly favored by artificial selection due to temperament and cunning respectively. Populations were found in equilibrium Hardy-Weinberg and showed excess of homozygotes and high levels of gene flow.

The objective of the study was to characterize microorganisms with probiotic potential of manure of lactating Brahman calves in Sucre (Colombia). Bacteria and yeasts were isolated from manure samples and the probiotic capacity of these strains was determined by bile salt resistance test (0.05, 0.1, 0.15, 0.3%), resistance to acidic pH (pH 3, 4, 5.6, 7 ), tolerance to NaCl (2, 4, 6, 8, 10%) and antagonistic activity (Salmonella sp and Escherichia coli). Nine microorganisms were identified and three passed the tolerance tests. The antagonistic capacity was determined against pathogenic bacteria (Salmonella sp and E. coli), evidenced by halos (mm) of the three selected strains. The identification of the strains was carried out by the biochemical methods API 50 CHL V5.1 for Lactobacillus and API 20 AUX for yeasts. Molecular analysis of two strains (M13a and 103M2) identified strain M13a as E...

The objective of this study was to investigate the genetic variability of criollo horses (Equus caballus) by genes associated with the coat. Fields visits were done between January and May 2016 in five populations of the municipality of Valencia, Colombia. The phenotypic characterization of the adult animals present in the farms was performed in relation to the autosomal markers of morphological codification Extension, Agouti, Cream, Gris, White, Tobiano, Overo and Roan. The results showed absence of the markers White and Overo, whereas the markers Extension and Agouti had the largest frequencies, possibly favored by artificial selection due to temperament and cunning respectively. Populations were found in equilibrium Hardy-Weinberg and showed excess of homozygotes and high levels of gene flow.

El objetivo del estudio fue caracterizar microorganismos con potencial probiótico de estiércol de terneros lactantes Brahman en Sucre (Colombia). Se aislaron bacterias y levaduras de las muestras de estiércol y se determinó la capacidad probiótica de estas cepas mediante pruebas de resistencia a sales biliares (0.05, 0.1, 0.15 y 0.3%), resistencia a pH ácido (pH 3, 4, 5.6, 7), tolerancia a NaCl (2, 4, 6, 8, 10%) y actividad antagónica (Salmonella sp y Escherichia coli). Nueve microorganismos fueron identificados y tres pasaron las pruebas de tolerancia. Se determinó la capacidad antagónica frente a bacterias patógenas (Salmonella sp y E. coli), evidenciada por halos (mm) de las tres cepas seleccionadas. La identificación de las cepas se realizó por los métodos bioquímicos API 50 CHL V5.1 para Lactobacillus y API 20 AUX para levaduras. El análisis molecular de dos cepas (M1...