Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)

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The aim of this study was to determine the prevalence of hepatic dystomatosis in cattle slaughtered in the Sogamoso slaughterhouse (Boyacá, Colombia), and to define the most effective diagnostic technique to establish the presence of the parasite. A sample size of 343 individuals was established con...

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Autores: Ortiz-Pineda, Melissa Camila, Archila-Barrera, Omar Alexander, Bulla-Castañeda, Diana María, Díaz-Anaya, Adriana María, Giraldo Forero, Julio Cesar, Garcia-Corredor, Diego José, Pulido-Medellín, Martín Orlando
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/21341
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21341
Nivel de acceso:acceso abierto
Materia:Fasciola hepatica
cattle
prevalence
ELISA
bovinos
prevalencia
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spelling Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)Diagnóstico post mortem de Fasciola hepatica en bovinos faenados en la planta de beneficio de Sogamoso (Boyacá, Colombia)Ortiz-Pineda, Melissa CamilaArchila-Barrera, Omar AlexanderBulla-Castañeda, Diana MaríaDíaz-Anaya, Adriana MaríaGiraldo Forero, Julio CesarGarcia-Corredor, Diego JoséPulido-Medellín, Martín OrlandoOrtiz-Pineda, Melissa CamilaArchila-Barrera, Omar AlexanderBulla-Castañeda, Diana MaríaDíaz-Anaya, Adriana MaríaGiraldo Forero, Julio CesarGarcia-Corredor, Diego JoséPulido-Medellín, Martín OrlandoFasciola hepaticacattleprevalenceELISAFasciola hepaticabovinosprevalenciaELISAThe aim of this study was to determine the prevalence of hepatic dystomatosis in cattle slaughtered in the Sogamoso slaughterhouse (Boyacá, Colombia), and to define the most effective diagnostic technique to establish the presence of the parasite. A sample size of 343 individuals was established considering the monthly slaughter rate of the plant. Stool, blood and bile samples were taken, and adult parasites were collected from the bile duct. The modified Ritchie technique was used in the coproparasitological examination, the bile content was analyzed by sedimentation and a standardized in-house ELISA was implemented for serological diagnosis from adult Fasciola hepatica parasites. The results showed that 29.7% (102/343) of the individuals sampled were positive for the presence of F. hepatica in at least one of the techniques used. The post mortem evaluation presented the highest value (19.8%; 68/343), followed by the in-house ELISA (13.7%; 47/343), detection of eggs in bile (117%; 40/343) and the coprological technique (7.3%; 25/343).El objetivo del estudio fue determinar la prevalencia de distomatosis hepática en bovinos faenados en la planta de beneficio de Sogamoso (Boyacá, Colombia), y definir la técnica diagnóstica más efectiva para establecer la presencia del parásito. Se estableció un tamaño muestral de 343 individuos teniendo en cuenta la tasa de sacrificio mensual de la planta. Se tomaron muestras de heces, sangre y contenido biliar y se colectaron los parásitos adultos del ducto biliar. En el examen coproparasitológico se utilizó la técnica de Ritchie modificada, el contenido biliar fue analizado mediante sedimentación y para el diagnóstico serológico se implementó un ELISA in house estandarizado a partir de parásitos adultos de Fasciola hepatica. El 29.7% (102/343) de los individuos muestreados fueron positivos a la presencia de F. hepatica en por lo menos una de las técnicas empleadas. La evaluación post mortem presentó el valor más alto (19.8%; 68/343), seguido por el ELISA in house (13.7%; 47/343), detección de huevos en bilis (117%; 40/343) y la técnica coprológica (7.3%; 25/343).Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2021-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2134110.15381/rivep.v32i5.21341Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 5 (2021); e21341Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 5 (2021); e213411682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21341/17307Derechos de autor 2021 Melissa Camila Ortiz-Pineda, Omar Alexander Archila-Barrera, Diana María Bulla-Castañeda, Adriana María Díaz-Anaya, Julio Cesar Giraldo Forero, Diego José Garcia-Corredor, Martín Orlando Pulido-Medellínhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/213412021-10-27T23:31:44Z
dc.title.none.fl_str_mv Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
Diagnóstico post mortem de Fasciola hepatica en bovinos faenados en la planta de beneficio de Sogamoso (Boyacá, Colombia)
title Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
spellingShingle Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
Ortiz-Pineda, Melissa Camila
Fasciola hepatica
cattle
prevalence
ELISA
Fasciola hepatica
bovinos
prevalencia
ELISA
title_short Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
title_full Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
title_fullStr Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
title_full_unstemmed Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
title_sort Post mortem diagnosis of Fasciola hepatica in cattle slaughtered at the Sogamoso, Boyacá processing plant (Boyacá, Colombia)
dc.creator.none.fl_str_mv Ortiz-Pineda, Melissa Camila
Archila-Barrera, Omar Alexander
Bulla-Castañeda, Diana María
Díaz-Anaya, Adriana María
Giraldo Forero, Julio Cesar
Garcia-Corredor, Diego José
Pulido-Medellín, Martín Orlando
Ortiz-Pineda, Melissa Camila
Archila-Barrera, Omar Alexander
Bulla-Castañeda, Diana María
Díaz-Anaya, Adriana María
Giraldo Forero, Julio Cesar
Garcia-Corredor, Diego José
Pulido-Medellín, Martín Orlando
author Ortiz-Pineda, Melissa Camila
author_facet Ortiz-Pineda, Melissa Camila
Archila-Barrera, Omar Alexander
Bulla-Castañeda, Diana María
Díaz-Anaya, Adriana María
Giraldo Forero, Julio Cesar
Garcia-Corredor, Diego José
Pulido-Medellín, Martín Orlando
author_role author
author2 Archila-Barrera, Omar Alexander
Bulla-Castañeda, Diana María
Díaz-Anaya, Adriana María
Giraldo Forero, Julio Cesar
Garcia-Corredor, Diego José
Pulido-Medellín, Martín Orlando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fasciola hepatica
cattle
prevalence
ELISA
Fasciola hepatica
bovinos
prevalencia
ELISA
topic Fasciola hepatica
cattle
prevalence
ELISA
Fasciola hepatica
bovinos
prevalencia
ELISA
description The aim of this study was to determine the prevalence of hepatic dystomatosis in cattle slaughtered in the Sogamoso slaughterhouse (Boyacá, Colombia), and to define the most effective diagnostic technique to establish the presence of the parasite. A sample size of 343 individuals was established considering the monthly slaughter rate of the plant. Stool, blood and bile samples were taken, and adult parasites were collected from the bile duct. The modified Ritchie technique was used in the coproparasitological examination, the bile content was analyzed by sedimentation and a standardized in-house ELISA was implemented for serological diagnosis from adult Fasciola hepatica parasites. The results showed that 29.7% (102/343) of the individuals sampled were positive for the presence of F. hepatica in at least one of the techniques used. The post mortem evaluation presented the highest value (19.8%; 68/343), followed by the in-house ELISA (13.7%; 47/343), detection of eggs in bile (117%; 40/343) and the coprological technique (7.3%; 25/343).
publishDate 2021
dc.date.none.fl_str_mv 2021-10-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21341
10.15381/rivep.v32i5.21341
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21341
identifier_str_mv 10.15381/rivep.v32i5.21341
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/21341/17307
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 5 (2021); e21341
Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 5 (2021); e21341
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
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repository.mail.fl_str_mv
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