Evaluation of the cellulase enzymatic complex in native fungi of Peru

• Autores: Llacza Ladera, Henry Frans, Castellanos Sánchez, Pedro Luis, Meza Mendoza, Patricia
• Formato: artículo
• Fecha de Publicación: 2021
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/21784
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/iigeo/article/view/21784
• Nivel de acceso: acceso abierto
• Materia: Enzymatic activity; Celulasas; Czapeck; Radial diffusion; Nelson-Somogyi; Actividad enzimática; Difusión radial; Somogyi-Nelson

In Peru, information on the biotechnological potential of native fungi for the degradation of cellulose presents few records. This work aimed to evaluate the cellulolytic activity of isolated fungi in the departments of Cajamarca, Lima, Junín and Huánuco. 289 species of fungi were subjected to reactivation after a conservation period of 7 years using filter paper and Czapeck broth, evaluated semi-quantitatively using the radial diffusion technique on Czapeck Na-CMC agar to then select five strains that were morphologically identified and its cellulolytic capacity was also determined by the Somogyi-Nelson method. 90% (260) of the fungi that underwent semi-quantitative evaluation were reactivated, selecting five strains for their greater clearance area: 14.9, 12.5, 12.0, 14.4 and 12.7 x 102 mm2; SA 726 (Paecilomyces sp), SA 668 (Paecilomyces sp), SA 651 (Paecilomyces sp), SA 683 (Fusarium sp) and HN 566 (Aspergillus sp) with EA of: 0.048, 0.042, 0.042, 0.080 and 0.048 IU / ml respectively. The native strains evaluated maintain good viability over time and retain the ability to degrade cellulose. These strains can be used in biotechnological processes for the treatment of agroindustrial effluents.