Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)

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The proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1,...

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Detalles Bibliográficos
Autores: Escobar, Enrique, Rivera, Carlos, Tincopa, Luz, Rivera, Dani
Formato: artículo
Fecha de Publicación:2002
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/2511
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2511
Nivel de acceso:acceso abierto
Materia:Toxina
Hadruroides lunatus
escorpión
veneno de escorpión
Toxin
scorpion
scorpion venom
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spelling Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)Purificación parcial de las toxinas HI1, HI2 y HI3 del veneno del escorpión Hadruroides lunatus Koch, 1867 (Scorpionida: Vejovidae)RPIONIDA: VEJOVIDAE)Escobar, EnriqueRivera, CarlosTincopa, LuzRivera, DaniToxinaHadruroides lunatusescorpiónveneno de escorpiónToxinHadruroides lunatusscorpionscorpion venomThe proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1, Hl2 y Hl3; which immobilize, respectively, insects (Grillus sp.), crustaceans (Porcellio laevis) and the inoculated limb of white mice. The three toxins are basic proteins and have no proteolitic or phospholipase activity. In addition, Hl3 increases the plasmatic level of creatine kinase after its inoculation in the gastrocnemius muscle of mice. By PAGE-SDS, Hl3 shows only one protein band of 12,5 KDa, while the other toxins have protein contaminants.Se han separado las proteínas del veneno del escorpión Hadruroides lunatus, por cromatografía de intercambio iónico en CM-Sephadex C-25 con buffer acetato de amonio 0,05M, pH 7, y se obtuvieron seis picos de proteína. Los ensayos de toxicidad de las fracciones colectadas han permitido identificar 3 toxinas que hemos denominado Hl1, Hl2 y Hl3, las cuales paralizan, respectivamente, insectos (Grillus sp.), crustáceos (Porcellio laevis) y la extremidad inoculada de ratones albinos. Las tres toxinas son de naturaleza básica, y carecen de actividad proteolítica y fosfolipásica. Adicionalmente, Hl3 aumenta los niveles plasmáticos de creatina kinasa, luego de ser inoculada en el músculo gastrocnemius de ratones albinos. Por electroforesis en gel de poliacrilamida con dodecil sulfato de sodio (PAGE-SDS), se ha determinado que Hl3 muestra una sola banda proteica de 12,5 KDa, mientras que las otras toxinas poseen contaminantes proteicos.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2002-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/251110.15381/rpb.v9i1.2511Revista Peruana de Biología; Vol. 9 Núm. 1 (2002); 3-10Revista Peruana de Biología; Vol. 9 No. 1 (2002); 3-101727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2511/2204Derechos de autor 2002 Enrique Escobar, Carlos Rivera, Luz Tincopa, Dani Riverahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/25112020-05-25T18:28:21Z
dc.title.none.fl_str_mv Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
Purificación parcial de las toxinas HI1, HI2 y HI3 del veneno del escorpión Hadruroides lunatus Koch, 1867 (Scorpionida: Vejovidae)RPIONIDA: VEJOVIDAE)
title Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
spellingShingle Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
Escobar, Enrique
Toxina
Hadruroides lunatus
escorpión
veneno de escorpión
Toxin
Hadruroides lunatus
scorpion
scorpion venom
title_short Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
title_full Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
title_fullStr Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
title_full_unstemmed Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
title_sort Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
dc.creator.none.fl_str_mv Escobar, Enrique
Rivera, Carlos
Tincopa, Luz
Rivera, Dani
author Escobar, Enrique
author_facet Escobar, Enrique
Rivera, Carlos
Tincopa, Luz
Rivera, Dani
author_role author
author2 Rivera, Carlos
Tincopa, Luz
Rivera, Dani
author2_role author
author
author
dc.subject.none.fl_str_mv Toxina
Hadruroides lunatus
escorpión
veneno de escorpión
Toxin
Hadruroides lunatus
scorpion
scorpion venom
topic Toxina
Hadruroides lunatus
escorpión
veneno de escorpión
Toxin
Hadruroides lunatus
scorpion
scorpion venom
description The proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1, Hl2 y Hl3; which immobilize, respectively, insects (Grillus sp.), crustaceans (Porcellio laevis) and the inoculated limb of white mice. The three toxins are basic proteins and have no proteolitic or phospholipase activity. In addition, Hl3 increases the plasmatic level of creatine kinase after its inoculation in the gastrocnemius muscle of mice. By PAGE-SDS, Hl3 shows only one protein band of 12,5 KDa, while the other toxins have protein contaminants.
publishDate 2002
dc.date.none.fl_str_mv 2002-07-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2511
10.15381/rpb.v9i1.2511
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2511
identifier_str_mv 10.15381/rpb.v9i1.2511
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2511/2204
dc.rights.none.fl_str_mv Derechos de autor 2002 Enrique Escobar, Carlos Rivera, Luz Tincopa, Dani Rivera
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2002 Enrique Escobar, Carlos Rivera, Luz Tincopa, Dani Rivera
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 9 Núm. 1 (2002); 3-10
Revista Peruana de Biología; Vol. 9 No. 1 (2002); 3-10
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238309265408000
score 13.95948
Partial purification of toxins HI1, HI2 and HI3 from Hadruroides lunatus Koch, 1867 scorpion venom (Scorpionida : Vejovidae)
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